摘要
为探寻黑豆皮花色苷(anthocyanins from black soybean seed coat,ABSC)抗禽白血病病毒A亚群(subgroup A avian leukosis virus,ALV-A)的活性效果。本文以ABSC粉为原料,采用高效液相色谱仪与质谱仪联机(HPLC-MS)的方法鉴定了其花色苷主要活性成分,随后采用体外实验,应用MTT法和观察细胞形态法检测了ABSC对鸡成纤维细胞系(DF-1)的细胞毒性,建立细胞模型,研究加入ABSC后对ALV-A的预防和治疗作用。结果表明:黑豆皮花色苷粉中含四种花色苷成分,其中主要为矢车菊色素-3-葡萄糖苷;ABSC对DF-1无细胞毒性的最大相对安全质量浓度为20μg/mL;ABSC在安全浓度范围内能抑制ALV-A的增殖,且抑制程度成剂量关系。结论,ABSC质量浓度为12μg/mL能够显著抑制ALV-A诱导的DF-1细胞形态变化,降低凋亡。
To study the inhibitory action of anthocyanins from black soybean seed coat （ABSC） to subgroup A avian leukosis virus （ALV-A） in vitro.ABSC powder was chosen as the raw material and its anthocyanins structure was identified by HPLC-MS.Then in vitro, MTT assay and cell morphological changes were used to observe the cytotoxic effect of ABSC on monolayer DF-] cells, and cell model was used to research ABSC＇s prevention and treatment roles in the ALV-A-infected different phase.The result showed that the extract of black soybean seed coat contained four major anthocyanins, the one of the main component was cyanidin-3-glucoside.When mass concentration of ABSC was less than 20μg/mL, DF-1 cells was well-grown without the cell toxicity. Introduced ALV-A to DF-1, ABSC could restrain the proliferation of ALV-A under the 20μg/mL, and inhibition effect was dose-response in the security concentration range.In summary, anthocyanins from black soybean seed coat of 12μg/mL had significantly inhibitory effect for DF-1 cell induced by ALV-A.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第7期344-349,共6页
Science and Technology of Food Industry
基金
国家自然科学基金资助项目(30972048)
北京市自然科学基金(重点项目6111001)
