摘要
[目的]采用D-酪氨酸荧光猝灭法建立同时测定6-苄基腺嘌呤和糠氨基嘌呤的定量分析方法。[方法]利用荧光偏振信号计算获得的荧光偏振度(p)和荧光各向异性(r)来区分6-苄基腺嘌呤和糠氨基嘌呤这2种天然植物性激素,同时利用荧光猝灭信号和双标准曲线计量分析法同时测定这2种天然植物性激素。[结果]D-酪氨酸-6-苄基腺嘌呤体系对应着较大的荧光偏振度和荧光各向异性度,而D-酪氨酸-糠氨基嘌呤体系则对应着较小的荧光偏振度和荧光各向异性度,因此通过计算其荧光偏振度和荧光各向异性可对这2种细胞分裂素的混合体系进行研究与表征。[结论]该法可以应用于市售李子和苦瓜样品表皮中6-苄基腺嘌呤和糠氨基嘌呤的同时测定,结果令人满意。
The method of simultaneous determination of 6-benzylaminourine and kinetin was established by D-Tyr fluorescence quenching.[Methods] The two natural exogenous cytokinins 6-benzylaminourine and kinetin were analyzed qualitatively by the degree of fluorescence polarization（p） and fluorescence anisotropy（r） based on the measurements of the polarized fluorescence signals,and quantified simultaneously based on the fluorescence quenching spectra signals and the double standard curve method.[Results] The degree of fluorescence polarization and fluorescence anisotropy of the D-Tyr-6-BA system were larger than that of the D-Tyr-KT system,and the two natural cytokinins mixed systems could be studied and characterized via calculating the degree of fluorescence polarization and fluorescence anisotropy.[Conclusions] The method was applied to simultaneous determination of 6-benzylaminourine and kinetin in plum skin and balsam pear skin with satisfactory results.
出处
《农药》
CAS
CSCD
北大核心
2013年第3期198-201,共4页
Agrochemicals
基金
国家自然科学基金项目(21175015)
重庆市教委科技项目(KJ121306)
关键词
荧光偏振
荧光猝灭
荧光各向异性
6-苄基腺嘌呤
糠氨基嘌呤
同时测定
fluorescence polarization
fluorescence quenching
fluorescence anisotropy
6-benzylaminopurine
kinetin
simultaneous determination
