摘要
研究了曙红Y(EY)的共振散射光谱、荧光光谱和吸收光谱,讨论了共振光散射与共振荧光的区别与联系。在EY水溶液三维荧光等高线光谱图中,瑞利散射线与荧光等高线有部分相交。EY的共振散射峰(5 2 5nm)介于荧光激发峰(5 1 4nm)和发射峰(5 3 6nm)之间。由光偏振实验,测得EY共振散射光谱5 2 5nm处的偏振度P =0 2 0。上述实验结果证明,EY的共振散射峰主要是共振荧光。在改变pH的实验中发现,EY共振光散射增强是由于酸碱平衡的移动导致荧光型体的形成。由于自吸收的影响,共振散射光强度与EY浓度之间不是严格的线性关系。
Resonance light scattering （RLS）, fluorescence and absorption spectra of eosine Y （EY） has been measured to study the difference and relationship between RLS and resonance fluorescence. In three-dimensional fluorescence contour spectra of EY aqueous solutions, Rayleigh scattering line intersects with fluorescence contour. The RLS peak at 525nm of EY lies just between fluorescence excitation peak （514nm） and emission peak （536nm）. In light polarization experiment, the polarization of RLS at 525nm was measured to be P=0.20. All the above experimental facts reveal that the RLS peak of EY mainly consists of resonance fluorescence. The mechanism of RLS enhancement with variation in pH is because of the formation of fluorescence species in acid-base equilibrium. Since light scattering is affected by light absorption, relationship between RLS intensity and EY concentration is not strictly linear.
出处
《化学通报》
CAS
CSCD
北大核心
2005年第8期628-632,共5页
Chemistry
基金
河北省自然科学基金资助项目 (2 0 0 15 3)
