摘要
目的:观察左卡尼汀注射液(Levocarnitine injection)对柔红霉素(Daunorubicin,DNR)所致的心肌细胞损伤的保护作用。方法:将培养5d的新生Wister乳鼠心肌细胞随机分为正常对照组、DNR损伤组和左卡尼汀低、中、高剂量保护组,加药后继续培养24 h,测定细胞存活率、乳酸脱氢酶(LDH)释放量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:左卡尼汀各实验剂量均能显著提高受损心肌细胞的存活率,抑制LDH释放量,减少MDA的生成,提高SOD活性。结论:左卡尼汀对柔红霉素所致的心肌细胞损伤具有保护作用。
Objective： This study aimed to determine the protective effect of levocarnitine on myocardial injury caused by daunorublcm （DNR）. Methods： Neonatal cardiomyocytes （aged 5 days） from trained Wister rats were randomly divided into the following groups： normal control group, low DNR injury group, and levocamitine protection group. High-dose DNR was administered and the cardiomyocytes were cultured for 24 h. Cell viability, lactate dehydrogenase （LDH） release, superoxide dismutase （SOD） activity, and malondialdehyde （MDA） content were determined. Results： The levocarnitine dose could significantly improve the survival rate of damaged myocardial cells, inhibit LDH release, and reduce MDA production and SOD activity. Conclusion： Levocamitine exhibited a protective effect on DNRiamycin-induced myocardial cell injury.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第4期195-197,共3页
Chinese Journal of Clinical Oncology
基金
2010年天津市卫生局科技基金项目(编号:2010KZ75)资助~~
关键词
左卡尼汀注射液
柔红霉素
心肌细胞
心脏毒性
levocamitine injection, daunorubicin, myocardial cells, cardiotoxicity
