摘要
目的 观察 5 氟尿嘧啶 (5 Fu)前体物质卡培他滨 (Capecitabine)对高转移潜能肝癌LCID2 0生长和转移的抑制作用。方法 用免疫组织化学方法检测 6 1例人肝癌组织中血小板衍化内皮细胞生长因子 (PD ECGF)蛋白水平。 18只裸鼠人肝癌高转移模型LCID2 0于肿瘤种植后第 3天分别采用CAP和 5 Fu治疗。停药后第 3天处死裸鼠 ,测量肿瘤的长短径 ,检测裸鼠肝功能和血浆甲胎蛋白 (AFP)水平 ;苏木素 伊红 (HE)染色检测肺转移 ;原位末端标记 (TUNEL)法检测肝肿瘤组织中的凋亡细胞。结果 6 1例肝癌和癌周组织中PD ECGF蛋白表达阳性率分别为 70 .5 %和 47.5 % ,高TNM分期组肝癌组织中PD ECGF蛋白表达阳性率 (81.1% )明显高于低TNM分期组表达率(5 4.2 % ) ,两者差异有显著性 (P <0 .0 5 )。CAP治疗后 ,裸鼠肝肿瘤体积 (2 40± 119)mm3 明显小于对照组 (4 47± 15 9)mm3 和 5 Fu治疗组 (4 42± 81)mm3(P <0 .0 5 ) ;血浆AFP水平明显下降 (P <0 .0 1) ;肺转移率显著降低 (P <0 .0 1) ;肝肿瘤组织中凋亡指数增大 (P <0 .0 1)。结论 CAP治疗能抑制高转移性人肝癌的生长和转移。
Objective\ To observe the inhibitory effect of Capecitabine(CAP),a new prodrug of 5-fluorouracil(5-Fu) on the growth and metastasis of LCID20, a highly metastatic hepatocellular carcinoma (HCC). Methods\ The protein level of platelet-derived endothelial cell growth factor (PD-ECGF) was determined by using immunohistochemical method in 61 cases of HCC. CAP and 5-Fu via oral administration were used in 18 nude mice bearing LCID20 tumor. All treatment lasted 3 weeks and the nude mice were killed 3 days after withdrawal of the drugs. The tumor size was calculated by using the formula: V=a×b 2×0.5. The AFP levels in the serum of the nude mice were detected by using radioimmunoassay. Lung metastasis was evaluated by HE staining in lung samples. The PD-ECGF staining and apoptosis in hepatoma were detected by using immunohistochemical method and TUNEL method respectively. Results The expression rate of PD-ECGF protein in 61 cases of HCC and paratumoral liver tissues was 70.5% and 47.5% respectively, and was higher in HCC tissues with advanced TNM stage (Stage I and II) than that in those with early TNM stage (Stage III and IV) (81.1% vs 54.2%, P <0.05). After the intervention by CAP, the tumor size (240±119) mm 3 was significantly smaller than that in the control (447±159) mm 3 and the 5-Fu group (442±81) mm 3 ( P <0.05); The AFP level was reduced markedly ( P < 0.01 ); The lung metastasis rate was decreased obviously ( P <0.01); In the tumor tissue,the intense of PD-ECGF staining weakened and the apoptotic cells were increased ( P <0.01). Conclusion\ PD-ECGF was highly expressed in HCC. CAP could obviously inhibit the growth and metastasis of highly metastatic HCC.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第5期403-405,I000,共4页
Chinese Journal of Experimental Surgery
基金
上海市百人计划基金!资助项目 (97BR0 2 9)
上海市科技发展基金!资助项目 (9844190 6 7)
关键词
肝细胞癌
PD-ECGF
卡培他滨
脱噬作用
Hepatocellular carcinoma
\ Platelet-derived endothelial cell growth factor
\ Capecitabine
\ Apoptosis
