摘要
目的观察高／低转移人肝癌细胞株MHCC97-H／L是否存在侧群（SP）细胞并鉴定其致瘤性。方法MHCC97-H／L予Hoechst33342和羟基荧光素二醋酸盐琥珀酰亚胺脂（CFSE）荧光染色；流式细胞分选MHCC97．H／L中SP细胞，分选后细胞皮下接种非肥胖性糖尿病联合免疫缺陷（NOD／SCID）小鼠，观察成瘤情况。结果Hoechst33342和CFSE染色结果显示，MHCC97-H／L中未染色细胞分别为（4．02±0．02）％／（1．02±0．01）％，流式细胞分选结果示MHCC97-H／L中sP细胞为（4．88±0．66）％／（0．88±0．36）％，比例差异有统计学意义（P〈0．05）。体内成瘤实验显示，SP细胞只需1×10。个即可在NOD／SCID小鼠皮下成瘤（5／6），而1×10^6个非SP细胞均未成瘤（0／6）。结论高／低转移人肝癌细胞株MHCC97-H／L中均存在SP细胞亚群，但前者比例显著高于后者。肝癌SP细胞具有极高的致瘤性，并可能和肝癌转移潜能相关。
Objective To identify the side population （SP） cells in hepatocellular carcinoma （HCC） cell line MHCC97-H/L and its tumor initiation. Methods MHCC97-H/L cell suspension was stained with Hoechst33342 and carboxyfluorescein diacetate succininidyl ester （CFSE）, and sorted by flow cytometry. Various numbers of SP and non-SP cells were transplanted to the male NOD/SCID. Tumor formation was monitored weekly. Results The percentage of SP cells was （4.02 ± 0.02）%/（ 1.02 ± 0.01 ） % in MHCC97-H/L respectively by Hoechst33342 and CFSE staining. The percentage of SP cells was （4.88 ±0.66） %/（0.88 ±0.36） % in MHCC97-H/L by flow cytometry. There was statistically significant difference （P 〈 0.05）. 1 × 10^4 SP cells were sufficient to tumor formation （5/6）, while 1 × 10^6 non-SP cells did not initiate tumors （0/6）. Conclusion MHCC97-H and MHCC97-L,which have different metastatic potentials, all contain SP cells. The percentage of SP cells in MHCC97-H, HCC cell line with high metastatic potential was significantly higher than MHCC97-L, HCC cell line with low metastatic potential. SP cells can initiate tumors more effectively and may be related to the metastatic potential of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第7期795-796,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(30500594)
关键词
癌
肝细胞
转移
复发
Carcinoma,hepatocellular
Metastasis
Recurrence
