摘要
目的:观察凉血通瘀方对出血性中风患者外周血中肿瘤坏死因子-α(TNF-α)、热休克蛋白70(HSP70)、NO和NOS的影响,探讨凉血通瘀方治疗出血性中风患者的作用及机制。方法:采集出血性中风患者的全血,培养加药,检测上清中TNF-α、HSP70的含量及NO浓度和NOS的活性。结果:加药组与对照组相比,TNF-α的含量下降,HSP70的含量升高、上清NO、NOS水平升高。结论:凉血通瘀方作用于出血性中风患者外周血可在一定程度上降低TNF-α的表达,升高HSP70的表达、影响NOS的活性和促进NO的分泌。这可能是凉血通瘀方治疗急性出血性中风的作用机理之一。
Objective：To observe the effect of Liangxue Tongyu prescription on expression of TNF-α,HSP70,NO and NOS in the peripheral blood of patients with hemorrhagic stroke and to study the mechanism of Liangxue Tongyu prescription in the treatment of hemorrhagic stroke.Methods：Peripheral blood of patients with hemorrhagic stroke was collected.It was cultivated and added drug.Supernatant TNF-α,HSP70 level was measured by using ELISA.The NO concentration and activity of NOS were detected by NO and NOS kits.Results：The results showed that under the effects of Liangxue Tongyu prescription on the peripheral blood of patients with hemorrhagic stroke,supernatant TNF-α level was decreased significantly after Liangxue Tongyu prescription treatment,supernatant HSP70 level was elevated significantly after Liangxue Tongyu prescription treatment and the NO,NOS levels were significantly elevated.Conclusion：To some extent,Liangxue Tongyu can reduce the expression of TNF-α level,increase the the expression of HSP70 and increase the activity of NOS to promote the secretion of NO in the peripheral blood of patients with hemorrhagic stroke.This may be one of the molecular mechanism of Liangxue Tongyu prescription in the treatment of hemorrhagic stroke.
出处
《中医药信息》
2013年第1期18-20,共3页
Information on Traditional Chinese Medicine
基金
国家科技部973计划基金资助项目(2006CB504807)
省教育厅基金资助项目(10KJB360005)
