摘要
目的通过离体细胞实验观察凉血通淤方对H2O2氧化损伤的血管内皮细胞(VEC)的保护作用。方法建立体外H2O2氧化损伤模型,采用MTT法观察凉血通淤方对VEC增殖的影响,流式细胞仪检测凉血通淤方对VEC的影响,并通过光镜和电镜进行形态学观察。结果 MTT结果显示凉血通淤方大剂量组OD值比H2O2组明显增高(P<0.01);光镜结果显示凉血通淤方大剂量组的细胞形态基本正常;电镜结果显示凉血通淤方大剂量组的细胞空泡明显减少。流式细胞仪检测结果显示凉血通淤方组的细胞碎片率较H2O2模型组明显降低。结论凉血通淤方可一定程度保护氧化损伤的血管内皮细胞。
Objective To observe the protective effect of Liangxue Tongyu Fang on vascular endothelial cell(VECs)injury induced by H2O2.Methods VECs were cultured in vitro,and an injured model induced by H2O2 was established.Cell viability was measured by MTT assay,the rate of cell debris was detected by flow cytometry(FCM),and the morphological changes of VECs were observed with the inverted microscope and electronic microscope.Results MTT results showed that OD valued in Liangxue Tongyu Fang group was significantly higher than that of H2O2 group(P<0.01).Light microscope results showed that Liangxue Tongyu Fang group had normal cell morphology.Electronic microscope results showed that high Liangxue Tongyu Fang group significantly reduced cell vacuoles.Flow cytometry results showed that the rate of cell debris in Liangxue Tongyu Fang group was significantly lower than that of H2O2 model group.Conclusion To some extent,Liangxue tongyu fang can protect VECs injury induced by H2O2.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第12期3064-3066,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家科技部"973"计划基金资助项目(No.2006CB504807)
