摘要
目的探讨UC001kfo对肝癌细胞侵袭的影响及调控目标是否为a-平滑肌肌动蛋白（a-SMA）。方法以肝癌细胞HepG2为细胞模型，分为UC001kfoRNA干扰组（UC001kfo—siRNA组）和阴性对照组，每组3个复孔，实时定量逆转录聚合酶链反应（RT—qPCR）检测沉默效率，细胞划痕试验和Transwell小室试验分析HepG2侵袭转移能力，RT—qPCR检测仅-SMAmRNA的表达，并将UC001kfo和d．SMA的表达量进行相关分析。结果划痕试验结果显示，阴性对照组、UC001kfo—siRNA组48h迁移距离分别为（17．55±0．44）um，UC001kfo-siRNA组（10．55±0．41）斗m，差异有统计学意义（P〈0．01）；Transwell小室侵袭试验结果显示，UC001kfo．siRNA组穿膜细胞数[（56±10）个]明显少于阴性对照组[（141±21）个，P〈0．01]；RT—qPCR结果显示UC001kfo—siRNA组UC001kfo相对阴性对照组的表达量为0．23±0．20，仪一SMA的表达量为0．36±0．17，与阴性对照组比较两者表达差异有统计学意义（P〈0．05），相关分析表明仪．SMA与UC001kfo的表达量呈正相关（r=0．997，P〈0．05）。结论UC001kfo通过调控仪．SMA的表达促进肝癌细胞的迁移侵袭。
Objective To study the impact of UC001kfo on the invasion of hepatocellular carcino- ma cells and whether the regulation target is ct-smooth muscle actin （ct-SMA）. Methods The hepatocel- lular carcinoma cell line HepG2 was used as the cell model, groups were divided into UC001 kfo RNA inter- ference group （UC001kfo-siRNA group） and negative control group. The RNA interference technique was performed to down-regulate the expression of UC001 kfo, then the real-time reverse transcriptase-polymerase chain reaction （RT-qPCR） was used to examine the silence efficiency, the scratch adhesion test and tran- swell small cell test were used to analyze the invasiveness of HepG2, RT-qPCR was used to examine the expression of ct-SMA mRNA, and the correlation analysis was conducted to the expressions of UC001kfo and ct-SMA. Results The 48 h migration distances of the negative control group and UC001kfo-siRNA group were （17.55 ±0. 44） ixm and （10. 55±0. 41 ） um respectively, and the difference has statistical significance （P 〈0.01 ） ; the number of transmembrane cells was （56 ±10） , which was significantly less than that of the negative control group of （ 141 ± 21 ） （ P 〈 0. 01 ） ; the RT-qPCR result shows that the UC001kfo relative expression of the UC001kfo-siRNA group was 0. 23 ＋ 0. 20, the expression of a-SMA was 0. 36 ±0. 17, and compared to the negative control group, both of them have statistical significance （P 〈 0. 05）. The correlation analysis shows that a-SMA has a positive correlation with the expression of UCOOlkfo （r =0. 997, P 〈 0. 05 ）. Conclusion UC001kfo can promote the migration and invasion of hepatocellular carcinoma cells by regulating ct-SMA.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第2期274-276,共3页
Chinese Journal of Experimental Surgery
