摘要
目的以结肠癌细胞SW480为模型,初步探讨Kiss-1基因过表达与结肠癌细胞增殖的关系。方法采用脂质体转染的方法将Kiss-1/pEGFP-N1重组质粒转染至SW480细胞,上调结肠癌细胞中Kiss-1的表达水平,经G418筛选出稳定表达株;根据细胞分为3组:阴性对照组(转染pEGFP-N1空载体)、实验组(转染pEGFPN1-Kiss1)及空白对照组(未转染)。Western blot检测转染后细胞中Kiss-1表达量的变化;采用CCK8试验分析Kiss-1对SW480细胞增殖的抑制效果。结果成功将pEGFP-N1-Kiss-1重组质粒转染至SW480结肠癌细胞,western blot检测出阴性对照组和空白对照组中显示Kiss-1低表达,实验组Kiss-1表达量增加,CCK8试验表明实验组细胞增殖率明显低于对照组(P<0.05)。结论 Kiss-1可抑制结肠癌细胞的增殖率。
Objective To use colon cancer cell SW480 as a model,to explore effects of overexpression of Kiss-1 on proliferation of colon cancer cells. Methods Using liposome transfection,Kiss-1 /pEGFP-N1 recombinant plasmid was transfected into SW480 cells,colon cancer Kiss-1 cells were upregulated,stably expressed cells were selected by G418; the cells were divided into 3 groups： negative control group（ pEGFP-N1 transfection with empty vector）,experimental group（ with pEGFP-N1-Kiss1） and control group（ without transfection）. Western blot detection was used to detect the expression of Kiss-1 after transfection and CCK8 test was applied to analyze the inhibitory effect of Kiss-1 on the proliferation of SW480cells. Results pEGFP-N1-Kiss-1 recombinant plasmid was successfully transfected into the colorectal cancer cell line SW480,Western blot detected low expression of Kiss-1 in the negative control group and blank control group,while Kiss-1in experimental group was increased; CCK8 test showed significantly lower rate of proliferation of cells in the experimental group than the control group（ P〈 0. 05）. Conclusion Kiss-1 can inhibit the proliferation of colon cancer cells rate.
出处
《中南医学科学杂志》
CAS
2014年第4期339-342,共4页
Medical Science Journal of Central South China
基金
湖南省教育厅一般项目(11C1107)
