摘要
目的观察肿瘤转移抑制基因Kiss1在结直肠腺瘤和结直肠癌的表达，探讨其临床意义。方法采用免疫组织化学及实时荧光定量聚合酶链反应（FQ-PCR）方法检测Kiss1在20例正常结直肠黏膜、30例结直肠癌以及80例结直肠腺瘤组织中的表达。结果Kiss1在正常结直肠黏膜、结直肠腺瘤及结直肠癌组织中表达水平的差异有统计学意义（P=0.005），阳性表达率分别为93.3%、58.8%、5.0%。在管状腺瘤、混合性腺瘤、绒毛状腺瘤组织中阳性表达率分别为80.0%、66.7%、35.0%，差异有统计学意义（P=0.005）。FQ-PCR检测结果显示在正常对照组、管状腺瘤组、混合性腺瘤组、绒毛状腺瘤组及结直肠癌组Kiss1表达量分别为28.85±8.34、14.61±1.65、7.58±1.31、2.26±1.56、0.50±0.40。结论Kiss1在结直肠腺瘤的恶变及结直肠癌的发生发展过程中起一定作用；Kiss1表达下调可能是结直肠腺瘤的癌变及结直肠癌发生的诱因。
ObjectiveTo investigate the expression and significance of Kiss1 protein in colon adenoma and colon carcinoma.MethodsImmunohistochemistry and real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） were used to detect the location and expression of Kiss1 in the 20 normal colon mucosae, 30 colon carcinomae, 80 colon adenomae.ResultsThe positive expression rate of Kiss1 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 93.3%, 58.8% and 5.0% respectively, with significant differences among them （P=0.005）. The positive expression rate of Kiss1 protein in tubular adenoma, mixed pattern of adenoma and villous adenoma was 80.0%, 66.7% and 35.0% respectively, with significant differences among them （P=0.005）. FQ-PCR showed that the Kiss1 mRNA expression rate was 28.85±8.34, 14.61±1.65, 7.58±1.31, 2.26±1.56 and 0.50±0.40 in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma, respectively.ConclusionKiss1 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma. Detecting the expression of Kiss1 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第5期866-868,共3页
Chinese Journal of Experimental Surgery
