摘要
目的探讨相对等量混合培养情况下不同血清型(O:3、O:9)小肠结肠炎耶尔森菌菌株是否存在竞争关系。方法将O:3、O:9菌株等量混合于100 mL Luria-Bertani(LB)培养液内作为实验组,另将O:3、O:9单独培养于100 mL LB培养液中作为对照组,28℃培养,每隔3 h取各组菌液涂板进行菌落计数,并以O:3单克隆抗体鉴定各菌落血清型。结果在混合培养条件下,O:3在生长峰值处的生长量均较单独培养时有所下降;在培养21、33、39 h时,O:3单独培养生长量分别是混合培养的4.40、5.81、4.79倍,在生长高峰阶段表现出明显抑制(P<0.05);混合培养对O:9生长量无明显影响;在生长速度方面,2种血清型在混合培养和单独培养条件下均未表现出明显差异。结论混合培养时,O:3对O:9的生长量无明显抑制作用,但O:9对O:3具有一定抑制作用。
Objective To investigate whether there is a competitive relationship between different serotypes of Yersinia enteroclitica（ Ye. enterocolitica strains O： 3, O： 9） by detecting the variation of colony-forming units （CFU） after mixture culture in vitro. Methods The Ye. enterocolitica strains of serotype O： 3 and O： 9 were mixed at the same quan- tity in 100 ml Luria-Bertani,meanwhile the two strains were cultured alone as two control groups. The oscillatory liquid were cultured at 28 ℃and the bacterial suspension of each group was cultured in the plate for colony counting every three hours. Then the monoclonal antibody was used to identify the serotype of each colony. Results The growth peak periods of O： 3 serotype in mixtured culture were significantly decreased than those cultured alone. At the culture time of 21,33, and 39 hours ,the bacterial counts of the O： 3 serotype strains were 4. 40,5.81, and 4. 79 folds higher than those cultured alone,respectively, and the growth peak perids of O ： 3 serotype were significantly inbibited by the O ： 9 serotype. However, no significant difference was observed between the O： 9 serotype strain cultured alone and mixed. The multipli- cation rate of the two serotypes did not show any difference in the mixed and cultured alone cultivation. Conclusion The O： 3 serotype did not inhibit the reproduction rate of O： 9. But the O： 9 serotylae could inhibit the O： 3 serotvoe.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第2期233-235,共3页
Chinese Journal of Public Health
基金
国家"十一五"传染病科技重大专项(2012ZXl0004-201)
