摘要
目的了解新平县家鼠鼠疫疫源地小肠结肠炎耶尔森菌的分布及病原学特征。方法采集家鼠盲肠、舌头和猪粪便、咽喉粘液以及腹泻患者粪便标本进行小肠结肠炎耶尔森菌的检测与分析。结果检测家鼠盲肠、鼠舌头、猪粪便、猪咽喉粘液物、腹泻患者粪便的标本数分别为722、722、467、237和107份,共分离到61株小肠结肠炎耶尔森菌,总检出率为2.71%,5种标本的检出率分别为2.63%、1.39%、3.85%、2.53%和7.48%,差异有统计学意义(χ2=16.422,P=0.003);分离株包括致病株10株、非致病株51株,有1A、2、3三种生物型和O:3、O:5、O:8等多种血清型,以及六种毒力基因型。猪、鼠、腹泻患者标本检出致病菌株数分别为9、1、0株。结论新平县家鼠鼠疫自然疫源地猪、鼠、腹泻患者是小肠结肠炎耶尔森菌的重要宿主,分离菌株具有遗传多样性,猪、鼠是小肠结肠炎耶尔森菌病的主要传染源。
Objective To understand the distribution and pathogenic characteristics of Yersinia enterocolitica in the foci of farmplague in Xinping county. Methods The caecum and tungue specimens of farm rat, the stool and throat secretion specimens of pigs andthe stool specimens of patients with diarrhea were collected and then Yersinia enterocolitica were cultured and tested. Results The specimens quantities of the caecum and tungue of farm rat, the stool and throat secretion of pigs and the stool of patients with diarrhea were722,722,467,237 and 107,respectively. 61 strains of Yersinia enterocolitica were isolated from all samples. The toal positive rate was2. 71% . The positive rates of five specimens were 2. 63%,1. 39% , 3. 85%,2. 53% and 7. 48%,respectively （x^2 = 16. 422,P = 0. 003）. The strains involved 10 pathogenic, 51 nonpathogenic or weak pathogenic strains, mainly concentrated in 1A, 2 and 3 biotypes ;0：3,0：5 and 0：8 serotypes; and six genotypes. The pathogenic strains quantities of pigs, rat and patients were 9,1 and 0,respectively. Conclusion The pigs,rat and patients with diarrhea in the foci of farm plague in Xinping county are the main hosts of Yersin-ia enterocolitica. The isoltes are of genetic diversities. The pigs and rat are the major infection sources of the relative diseases.
出处
《中国微生态学杂志》
CAS
CSCD
2013年第10期1123-1127,共5页
Chinese Journal of Microecology
基金
国家科技重大专项课题(2009ZX10004-201
2012ZX10004201)
关键词
小肠结肠炎耶尔森菌
宿主
传染源
毒力基因
Yersinia enterocolitica； Host； Infection source； Virulence genes
