摘要
目的通过研究噻托溴铵对慢性阻塞性肺疾病（COPD）大鼠血清及肺组织中肺表面活性蛋白-D（pulmonary surfaetant proteinD，SP—D）水平的影响，探讨噻托溴铵治疗COPD的新机制。方法采用烟熏加气管内注入内毒素法建立大鼠COPD模型。将雄性SD大鼠随机分为对照组、COPD组和噻托溴铵干预组，每组10只。HE染色观察大鼠肺、支气管的病理改变，测定肺平均内衬间隔及平均肺泡数。酶联免疫吸附试验检测各组大鼠血清中SP—D的含量。Westernblot及免疫组织化学方法检测大鼠肺组织中SPD的含量变化。结果COPD组大鼠肺、支气管的HE染色符合COPD的病理改变；COPD组大鼠出现明显肺气肿，噻托溴铵组也出现肺气肿，但程度较轻。COPD组大鼠血清及肺组织中SP—D水平较对照组均明显升高（P〈0．01），噻托溴铵丁预组与COPD组相比血清及肺组织中SP—D水平均显著降低（P〈0．01），但仍明显高于对照组（P〈0．01）。结论噻托溴铵可以降低COPD大鼠血清及肺组织中SP—D水平．这一作用可能是通过减轻气道炎症及肺气肿的程度来实现的。
Objective To investigate ~he new mechanism of tiotropium bromide in the treatment of chronic obstructive puhnonary disease （COPD） by studying its effect on pulmonary surfaetant protein D （SP-D） in serum and lung tissue of COPD rats. Methods We established the rat COPD models by passive smoking as well as intratracheal instillation of I.ipopolysaceharide respectively. Thirty male SD rats were randomly divided into the control group, the COPD group and the tiotropium bromide treatment group （n = 10）. The pathologic change of the lung tissue and airway were observed by HE staining. The level of SP I） in serum was measured by enzyme-linked immunosorbent assay. The level of SP-D in lung tissue was detected by western blot and immunohistochemistry. Results HE staining proved that the rat COPD models were successfully established. The COPD group appears obvious emphysema, while the treatment group appears mild emphysema. The expression of SP-D in serum and lung tissue of the COPD group were obviously stronger than that in the control group （ P 〈0. 01）. The level of SP-D in serum and lung tissue of the treatment group was between the control group＇ s and the COPD group＇ s. Conclusions Tiotropium bromide could reduce the level of SP-D in serum and lung tissue of COPD rat. These effects may be exerted through alleviating airway inflammation and the degree of emphysema.
出处
《国际呼吸杂志》
2013年第3期180-184,共5页
International Journal of Respiration
关键词
慢性阻塞性肺疾病
噻托溴铵
肺表面活性蛋白-D
Chronic obstructive puhnonary disease
Tiotropium bromide
Pulmonary surfactant protein 1）
