摘要
目的比较本研究所制备的流感病毒裂解疫苗儿童剂型(昆明疫苗)与赛诺菲巴斯德流感裂解疫苗儿童剂型(巴斯德疫苗)在动物中的安全性和免疫效果。方法用流感病毒裂解疫苗儿童剂型于0d和14d,腹腔内接种ICR小鼠。每天称取体质量,免疫后35d,眼眶采血分离血清,用微量血凝抑制(HI)法测定小鼠血清中特异性抗体滴度,计算抗体阳转率、保护率及几何平均滴度(GMT)增长倍数。结果免疫后小鼠体质量逐渐增加,组间无明显差异;两组间HI抗体阳转率、保护率及H1N1、H3N2型和B型HI抗体倍数差异无显著意义。结论两种疫苗均具有很好的安全性和免疫原性。
Objective To compare the safety and immunity of imported influenza vaccine and native influenza vaccine in mice.Methods The ICR mice were inoculated with split influenza virus vaccines on 0 d and 14 d,and were weighed everyday.The serum were separated after first-dose of two vaccines on 35 d.HI method was performed to etermine the specific serum antibody titers,as well as the increasing times of mean geometric titer in HI were analyzed.Results The statistical analysis indicated that growth of mice weight were not significantly different.Meanwhile,there was also no significant difference between two groups for seroconversion and protective level.Conclusion Immune effects of the two kinds of vaccines in serology might be almost as the same,and the safety and immunogenicity might be both satisfied.
出处
《国际检验医学杂志》
CAS
2012年第15期1793-1794,1797,共3页
International Journal of Laboratory Medicine
基金
国家863资助课题(2006AA02Z409)
云南省自然科学基金资助项目(2004C0720M
2006YX29)
关键词
流感病疫苗
药物评价
免疫原性
剂型
儿童
influenza vaccines
drug evaluation
immunogenicity
dose forms
chlid
