摘要
烟草病毒病发生普遍,在我国部分烟区已上升为烟草的第一大病害。烟草是云南省主要经济支柱之一,然而,病毒病的发生严重影响烟叶的产量和质量,造成一定经济损失。20世纪80~90年代,我国系统地调查了全国烟草侵染性病害,并对侵染我国烟草的病毒种类和严重影响生产的主要种类进行鉴定检测。
The total 73 different tobacco virus disease samples collected from different regions in Yunnan were detected with electron microscopy, DAS-ELISA, immunostrip test and multiplex RT-PCR. The results showed that the tobacco virus diseases were serious in Yunnan Province. 10 kinds of viruses were detected in the experiments. The viruses were more at the northeast and central part, such as Chuxiong and Wenshan, than the north part of Yunnan. The results showed that main viruses were Tobacco mosaic virus( TMV), Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tobacco etch virus (TEV) and Tobacco vein banding mosaic virus(TVBMV). The detection results showed there were nine types complex infections. The complex infection sample was 31.50% among the total testing samples. The main type of complex infection was that TEV and TVBMV, occupied 34.78% among the all combination.
出处
《植物病理学报》
CAS
CSCD
北大核心
2013年第1期100-103,共4页
Acta Phytopathologica Sinica
基金
中国烟草总公司云南省公司科技计划项目(No.2010YN18)