摘要
本研究利用多重RT-PCR技术,对葡萄的检疫性病毒中番茄环斑病毒(ToRSV)和烟草环斑病毒(TRSV)进行检测, 并研究了反应体系中各个参数对多重RT-PCR的影响。结果表明:可以在同一反应体系中实现对ToRSV和TRSV的RT- PCR检测,但模板浓度、引物浓度、循环数等3个因素对检测结果的准确性有较大的影响,3个参数的增加都会导致非特异性扩增的增多,而dNTP以及Taq酶的浓度对实验结果并无较大影响。
Multiple RT-PCR was applied to detect the import quarantine viruses on grapevine: Tomato ring-spot virus (ToRSV), Tobacco ringspot virus (TRSV). The factors affecting RT-PCR reaction of this system were studied,too. The results indicated ToRSV and TRSV could be detected by this method successfully, and three ingredients; template concentration, primer concentration and cycles had more influences on the results in the system than that of dNTP concentration and Taq DNA polymerase concentration.
出处
《植物病理学报》
CAS
CSCD
北大核心
2005年第S1期14-17,共4页
Acta Phytopathologica Sinica
