摘要
目的探讨乙型肝炎患者血清标志物与HBV-DNA定量检测之间的关系及其临床意义。方法选择258例经我院确诊的乙肝患者,采用酶联免疫吸附试验法检测患者体内血清标志物,采用荧光定量聚合酶链反应检测HBV-DNA含量,比较血清标志物与HBV-DNA检测量之间的关系。结果 HBsAg+/HBeAg+组与HBsAg+/HBeAg+/抗-HBc+组HBV-DNA阳性检出率与含量最高;HBsAg+/抗-HBe+/抗-HBc+、HBsAg+/HBc+、抗-HBs+/抗HBe+/抗-HBc+、抗-HBe+/抗-HBc+、抗-HBs+5种感染模式下HBV-DNA阳性检测率分别为62.96%、52.94%、37.50%、27.27%、4.35%。结论乙肝患者血清HBV-DNA阳性率与血清标志物存在相关性;同时进行血清标志物的检测与HBV-DNA定量检测能较准确直接地反应病毒复制程度及传染强度,对于早期诊断、治疗及预后判断具有指导意义。
Objective To investigate the correlation of serum markers and HBV-DNA levels in hepatitis B patients and its clinical significance. Methods Two hundred and fifty-eight hepatitis B patients was detected for serum markers by ELISA and HBV-DNA levels by quantitative fluorescent polymerase chain reaction （QF-PCR）.The correlation between serum markers and HBV-DNA levels were investigated. Results In HBsAg＋/HBeAg＋ group and HBsAg＋/ HBeAg＋/anti-HBc＋ group, the positive rate of HBV-DNA and the levels of HBV-DNA were the highest. For the five infection modes of HBsAgTanti-HBe＋/anti-HBc＋, HBsAg＋/HBc＋, anti-HBsTanti-HBe7 anti-HBc＋, anti-HBe＋/anti-HBc＋, anti-HBs＋, the HBV-DNA positive rates were 62.96%, 52.94%, 37.50%, 27.27%, 4.35%, respectively. Conclusion There is a correlation between the positive rate of HBV-DNA and the serum markers in hepatitis B patients. The detection serum markers combined with the quantitative detection of HBV-DNA can accurately reflect the extent of virus replication and infection intensity, which is of great significance for the early diagnosis, treatment and prognosis of hepatitis B.
出处
《海南医学》
CAS
2013年第1期49-50,共2页
Hainan Medical Journal
