摘要
目的:研究细毡毛忍冬总黄酮和绿原酸的聚酰胺同时富集工艺。方法:以紫外分光光度法测定其样品中总黄酮含量,HPLC测定绿原酸含量为指标,考察多个参数。结果:聚酰胺对细毡毛忍冬总黄酮和绿原酸有良好的吸附作用,其吸附洗脱工艺:上样溶液浓度0.04 g生药/mL,pH 5～6(原液),以1.5 mL/min的吸附流速上样,再依次用10%、50%乙醇对吸附后的聚酰胺进行洗脱,洗脱体积200 mL,洗脱剂pH值9～10。结论:聚酰胺可同时用于细毡毛忍冬总黄酮和绿原酸的分离富集。
Objective：To select the optimal condition of polyamide column adsorption chromatogtaphy for separating and enriching the total flavonoids and Chlorogenic Acid from Lonicera similes Hemsl.Methods：Total flavonoids content,in the sample solution of Lonicera similes Hemsl,detected by UV spectrophotometry,at the same time,Chlorogenic Acid detected by HPLC.Results：Polyamide has good absorbing effect on total flavonoids and Chlorogenic Acid.The liquid concertration of its absorbing and separation technological condition is about 0.04 g·mL-1 herbal extract,pH 5～6 and the absorbing speed of 1.5mL·min-1.The elution effect of 10% and 50% alcohol in turn,200 mL separately,pH 9～10 is best.Conclusion：The method is effective,fit for total flavonoids and Chlorogenic Acid from Lonicera similes Hemsl.
出处
《成都中医药大学学报》
2012年第4期52-54,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省教育厅重点项目(编号:2010A036)
关键词
细毡毛忍冬
聚酰胺
总黄酮和绿原酸
Lonicera similes Hemsl
polyamide
total flavonoids and Chlorogenic Acid
