摘要
本研究对多糖产生菌株sp.54A-42进行了16SrDNA序列比对分析,鉴定其为枯草芽孢杆菌。对发酵所得多糖产物进行了薄层层析、高效液相色谱、红外光谱和核磁共振分析,证明发酵产物为左聚糖(β-2,6-果聚糖)。通过紫外-LiCl复合诱变方式选育,得到突变株sp.Z-49,多糖产量达到16.1g/L,比出发菌株提高了34.2%,经5次传代发酵实验产量稳定,为进一步研究左聚糖的发酵生产打下了良好基础。
The polysaccharides-producing strain sp.54A-42 was identified to be Bacillus subtilis based on deter-mination and alignment of its 16S rDNA sequence. Its fermenting polysaccharides were proved to be levan （β-2,6-fructan） through the biophysical analysis of thin layer chromatography, high performance liquid chro-matography, infrared spectroscopy and nuclear magnetic resonance. By using the approach of ultraviolet-LiCl compound mutagenesis, the mutant sp.Z-49 was obtained, and the polysaccharides yield of this mutant reached 16.1 g/L with increase of 34.2% compared with the starting strain, which remains a stable yield after five inoculm passages. This research may lay a good foundation for further study on levan producing by fermentation.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2012年第6期567-573,共7页
Genomics and Applied Biology
基金
国家自然科学基金(31060125)资助
关键词
枯草芽孢杆菌
左聚糖
突变选育
生物物理鉴定
Bacillus s ubtilis, Levan, Mutagenesis breeding, Biophysical characterization
