摘要
通过单因素试验(培养基用水、碳源、氮源、培养温度和培养基初始pH值)和正交试验对地衣芽孢杆菌(Bacillus licheniformis)8-37-0-1发酵产生Levan果聚糖的培养基组成及培养条件进行优化,采用苯酚-硫酸法测定多糖含量。结果表明:以蔗糖100g/L、牛肉膏1.0g/L、酵母粉0.6g/L、K2HPO4 3.0g/L、KH2PO4 3.0g/L、NaCl1.0g/L、MgSO4.7H2O 0.2g/L、FeSO4.7H2O 0.001g/L,自来水配制,培养基初始pH5.0,30℃培养8-37-0-1菌株24h,Levan果聚糖产量达到最高值41.7g/L,约是未优化时的5.0倍。
The medium and fermentation conditions for levan production by Bacillus licheniformis 8-37-0-1 were optimized by single factor and orthogonal array experiments.After culture at 30 ℃ for 24 h in a medium prepared with tap water consisting of sucrose 100 g/L,yeast extract 0.6 g/L,beef extract 1.0 g/L,KH2PO4 3.0 g/L,K2HPO4 3.0 g/L,NaCl 1.0 g/L,MgSO4·7H2O 0.2 g/L and FeSO4·7H2O 0.001 g/L,a maximum levan production of 41.7 g/L was achieved,which was 5.0 times higher than before optimization.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第7期183-187,共5页
Food Science
基金
国家自然科学基金项目(31070064)
山东大学自主创新基金项目(2009DX002)
