摘要
竹叶粉碎后经过高温水提醇沉得竹叶多糖粗品。用DEAE琼脂糖凝胶FF和丙烯葡聚糖凝胶S-100分离纯化出两种均一多糖BLP30-1、BLP30-2,并对这二种多糖进行组分分析。气相分析结果表明,两者都由岩藻糖、木糖、甘露糖、葡萄糖、半乳糖五种糖组成。采用尺寸排除色谱和激光光散射联用仪(SEC-LLS)测定分子量分别为2.9×104、2.1×104u。对两种多糖进行抗氧化活性研究表明,其对DPPH自由基、超氧阴离子自由基、羟基自由基均有较高的抑制活性。
Crude polysaccharide was extracted by hot water and precipitated by ethanol. Two purified polysaccharides were obtained by DEAE Sepharose FF and Sephcryl S -100,components of BLP30 -1 and BLP30 -2 were studied by GC method. The result showed that they were all composed of fucose,xylose, mannose,glucose,galactose. The molecular weight of the polysaccharides was obtained by SEC -LLS and their molecular weight were 2.9×10 4 u and 2.1×10 4 u,respectively. Available data obtained with in vitro models suggested that they showed significant inhibitory effects on DPPH radical,hydroxyl radical,superoxide radical.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第2期100-103,共4页
Science and Technology of Food Industry
基金
国家"十二五"科技支撑项目(2011BAD23B02)
浙江省重大科技专项农业项目(2008C12036)
浙江省首批重点创新团队项目(2009R50028)
浙江省重点实验室开放基金(2010KF0506)
浙江科技学院科研启动基金资助项目(F501103906)
关键词
竹叶
多糖
组成
抗氧化活性
Bamboo-Leaf
polysaccharides
composition
antioxidant activities
