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响应面法优化微波辅助提取竹叶多糖工艺研究 被引量:5

Optimization of microwave-assisted extraction of polysaccharides from bamboo leaves by response surface analysis
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摘要 利用微波辅助技术提取竹叶多糖。在单因素试验的基础上,运用响应面分析法,研究液固比、提取时间、提取温度对竹叶多糖提取率的影响,建立多糖提取得率的二次回归方程,并确定了竹叶多糖的最佳提取工艺条件为:微波功率为600 W,微波提取温度124℃,提取时间44 min,液固比41:1,采用该工艺条件,提取1次,竹叶多糖的提取率达到0.45%。而理论预测多糖得率是0.456%,实际得率达到理论预测值的98.68%。 The extraction of polysaccharides from Neosinocalamus affinins was studied in this paper. Ac- cording to the single factor experiment, three levels of the factors (extraction temperature, time and the ratio of liquid-to-solid) were selected for the Box-Behnken factorial design to establish a quadric regression equation for describing the yield of polysaccharides. By response surface analysis, the optimal extraction conditions were obtained as follows: extraction power of 600 W, extraction temperature of 124~C, extraction time of 44 min and liquid-to-solid ratio of 41:1, under which the experimental yield (0.45%) of polysaccharides accounts for 98.68% of the theoretically predicted value of 0.456% with one-time extraction.
出处 《安徽农业大学学报》 CAS CSCD 北大核心 2012年第3期401-406,共6页 Journal of Anhui Agricultural University
基金 林业公益性行业科研专项(20100400708) 国际竹藤网络中心基本科研业务费项目(1632010003)共同资助
关键词 慈竹 总多糖 微波辅助提取 响应面法 Neosinocalamus affinins total polysaccharides microwave-assisted extraction (MAE) responsesurface analysis (RSA)
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