摘要
以小麦－中间偃麦草二体附加系L1衍生抗病系PP9－1为抗源，与小麦推广品种陕7859、丰抗8号杂交并自交，在F6代中选到农艺性状优良的高抗黄矮病小麦新品系YW43。对YW443及其亲本进行抗病性鉴定。结果表明：YW443高抗大麦黄矮病毒GPV、GAV株系。利用基因组原位杂交、RFLP分析和RAPD分析，研究YW43的遗传构成及其抗病基因染色体归属。结果表明：YW443（2n＝42）的遗传构成为40条（20对）小麦染色体和2条（1对）小麦－中间偃麦草易位染色体，易位的中间偃麦草染色体片段较小，属于St基因组，小麦染色体7D长臂末端片段被中间偃麦草染色体7St长臂末端片段取代，小麦新品系YW443为小麦－中间偃麦草7DS·7DL－7StL易位系，其抗病基因位于7StL末端。筛选出的特异RAPD标记OPR19-900，能检测出L1及其衍生抗病系的中间偃麦草染色体7StL片段，可作为鉴定抗黄矮病易位系的1个标记。
A new wheat line YW443 with BYDV resistance andgoodtraitswasdevelopedfromthecombination of PP9-1/Shan7859/Fengkang 8. The wheat line YW443 was identified by BYDV resistance analysis, genomic in situ hybridization (GISH), RFLP and RAPD analysis. The results indicated as follows: (1) YW443 was resistant to GPV and GAV strains of BYDV; (2) YW443 is a homozygous wheat-Thinopyrum intermedium translocation line with a pair of BYDV resistance genes; (3) The 7StL segment of Th. intermedium carring BYDV resistance gene was transferred onto the distal end of the wheat chromosome 7D long arm, the line YW443 was 7DS. 7DL-7StL translocation; (4) A RAPD marker OPR19-900 could detect the DNA of Th. intermedium 7StL in L1 and L1 derivatives including translocation lines YW443, YW642 etc. The markers may be used as a selecting marker for the BYDV resistance breeding program.
基金
国家863高技术项目!863-Z17-05-01
关键词
小麦
YW443
黄矮病
抗病品系
抗病基因
分子鉴定
barley yellow dwarf virus (BYDV)
Thinopyrum intermedium
translocation lines
genomic in situ hybridization (GISH)
RFLP
RAPD
