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大麦黄矮病毒GAV株系外壳蛋白基因在大肠杆菌中的高效表达 被引量:2

High-level Expression of BYDV GAV Coat Protein Gene in Escherichia coli
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摘要 The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a. The IPTG inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E.coli BL21(DE3).High level expression of the specific protein was achieved by IPTG induction.The results of SDS PAGE and Western blottong show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET 5a.
出处 《生物工程学报》 CAS CSCD 北大核心 2000年第3期400-402,共3页 Chinese Journal of Biotechnology
基金 国家攀登计划!( 85 3 1) 植物病虫害生物学国家重点实验室开放课题资助项目!( 98PD0 9 99PD0 3 )
关键词 大麦黄矮病毒 外壳蛋白基因 大肠杆菌 基因表达 BYDV GAV,coat protein gene,expression
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  • 1周广和 张淑香 等.小麦黄矮病毒4种株系鉴定与应用[J].中国农业科学,1987,20(4):7-12. 被引量:10
  • 2成卓敏 周广和.小麦黄矮病毒GPV株系的提纯及血清学研究[J].病毒学报,1986,2:275-277. 被引量:7
  • 3周广和 张淑香.一种由麦二叉蚜和麦长管蚜传播的小麦黄矮病毒株系的鉴定[J].植物病理学报,1986,16(1):17-21. 被引量:3
  • 4周广和,中国农业科学,1987年,20卷,7页 被引量:1
  • 5成卓敏,病毒学报,1986年,2卷,275页 被引量:1
  • 6周广和,植物病理学报,1986年,16卷,17页 被引量:1

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