摘要
目的通过线栓法制备大鼠慢性后肢缺血模型,将其与急性后肢缺血模型进行比较研究。方法采用线栓法制备Lewis大鼠慢性后肢缺血模型,分别于术后第7、14、28、42及49天进行激光多普勒血流分析及血管造影。每组动物在血管造影后处死,分别取其健侧和患侧股四头肌和腓肠肌行HE染色及α-actin免疫组织化学染色,并计算小动脉密度。结果慢性后肢缺血模型组动物术后未出现明显的跛行和肢体坏死。血流分析发现,慢性后肢缺血模型在术后第49天仍处于缺血状态。后肢肌肉组织病理学检查未发现急性坏死和肌肉纤维化的表现。术后第7天,慢性缺血组股四头肌的小动脉密度低于急性缺血组(0.015 2比0.036 4)。结论线栓法制备大鼠慢性后肢缺血模型与目前采用的急性动物后肢缺血模型制备方法有显著的不同,其缺血肢体较少受到代偿机制的影响且缺血时间维持较长,此为进一步研究缺血后血管新生的机理和治疗严重的下肢缺血提供了一种新的动物模型。
Objective To establish chronic hindlimb ischemia model with suture-occluded method in rats,and then compare the effects of chronic hindlimb ischemia model with acute ischemia model.Methods Models of chronic hindlimb ischemia were established by using suture-occluded femoral artery method.The laser Doppler blood flow analysis and angiography were performed on day 7,14,28,42,and 49 after operation,and then the rats were sacrificed after angiography,respectively,the quadriceps and gastrocnemius of contralateral and ipsilateral（surgical side） were gotten,which were tested by HE staining and α-actin immunohistochemistry staining,and then calculate arteriolar density.Results There were no lameness and limb necrosis after operation in chronic hindlimb ischemia models.Laser Doppler analysis found that chronic hindlimb ischemia models were still maintained in ischemia state on day 49 after operation compared with acute ischemic models.The resluts of HE staining showed no acute necrosis and muscle fibrosis in chronic hindlimb ischemia model group.Chronic hindlimb ischemia models after operation did not appear obvious lameness and limb necrosis.The arteriolar density of quadriceps femoris on day 7 after operation in chronic hindlimb ischemia models were less than that in acute hindlimb ischemia models（0.015 2 vs.0.036 4）.Conclusion sCompared with the commonly used acute ischemic models,the duration of arterial limb ischemia in chronic hindlimb ischemia rats,which were established by suture-occluded method,is longer and less likely to be affected by the compensatory mechanisms.So suture-occluded method can provide a new animal hindlimb ischemia model for further study of ischemia angiogenesis mechanism and treatment of severe lower extremity ischemia.
出处
《中国普外基础与临床杂志》
CAS
2012年第12期1291-1297,共7页
Chinese Journal of Bases and Clinics In General Surgery
基金
首都医科大学基础临床合作课题(项目编号:2006JK62)
北京市教委科技创新平台(项目编号:PXM2008-014226-067275)~~
关键词
慢性下肢缺血
线拴法
大鼠
动物模型
Chronic lower limb ischemia
Suture-occluded method
Rat
Animal model
