摘要
用线栓和结扎股动脉两种方法制备SD大鼠下肢缺血模型,通过多普勒激光扫描记录各组双下肢血流灌注值(PU),取大鼠双后肢腓肠肌及线栓处股动脉进行HE染色;手术侧腓肠肌,采用Western blot法检测血管内皮生长因子(VEGF)和血小板-内皮细胞黏附分子-1(CD31)。结果显示,结扎组的组织炎症、水肿发生时间较线栓组提前,而组织血流灌注在第49天时两组并无太大差异,结扎组的VEGF和CD31变化时间均比线栓组提前,但两者的变化趋势是一样的,在第49天时除线栓组CD31外,均可恢复正常水平。长时间观察两种模型并没有太大差异,故两种方法都可制作慢性下肢缺血模型。
Male SD rats were divided into ligation induced hind-limb ischemia group,suture-occlusion induced hind-limb ischemia group,sham operation group respectively. Ligation group was subjected to ligate the femoral artery,the occlusion group was occluded the femoral artery by suture. The laser Doppler imager was performed to evaluate the blood flow at different time points after operation. Then,the rats were sacrificed and the gastrocnemius and vessel were achieved to be further examined after angiography. Western blot was used to detect the protein expression of VEGF and CD31. Typical pathological manifestation of hind-limb ischemia was found in both experimental models,such as inflammation infiltration,edema and muscle fibrosis. There was no obvious difference of the blood flow in the two experimental groups. Western blot results showed that the protein expressions of VEGF and CD31 were decreased at early time of ischima,then gradually restored till on the 49 th day,which indicated that similar proteins changes happened in two ischima groups. There was no obvious difference in two ischemia models,so the two methods could be used to induce chronic hind-limb ischemia animal model successfully.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第6期913-916,共4页
Acta Universitatis Medicinalis Anhui
基金
四川省卫生计生委2014年科研基金(编号:140035)
关键词
结扎法
线栓法
下肢缺血模型
ligation
embolization
hindlimb
ischemia model
