摘要
目的探讨亚砷酸钠对正常人Chang肝细胞体外增殖及凋亡的影响。方法采用0(对照)～100μmol/L亚砷酸钠分别处理细胞24、48、72、96 h。采用MTT比色法检测细胞活力;采用流式细胞术检测染毒48 h时的细胞凋亡率。结果仅0.2μmol/L亚砷酸钠染毒24 h的细胞活力略高于对照组,但差异无统计学意义;且随着亚砷酸钠染毒浓度的升高和染毒时间的延长,Chang肝细胞存活率均呈下降趋势,并具有剂量-效应和时间-效应关系。与对照组相比较,5～100μmol/L亚砷酸钠染毒组Chang肝细胞凋亡率均显著升高,差异有统计学意义(P<0.05);且随着亚砷酸钠染毒浓度的升高,Chang肝细胞的凋亡率呈显著升高趋势,并具有剂量-效应关系。结论亚砷酸钠有明显的细胞毒性,能抑制Chang肝细胞的增殖,这可能与其诱导细胞凋亡有关。
Objective To explore the effect of sodium arsenite on the proliferation and apoptosis of human hepatocyte cell line Chang in vitro. Methods After the Chang liver cells were treated with different concentrations of sodium arsenite （0-100 μmol/L） for 24, 48, 72 and 96 h in vitro, the proliferation of Chang liver cells was determined by MrI＇F assay; The apoptosis rate of Chang liver ceils in 48 h was identified by flow cytometry assay, Results Only the activity of Chang liver ceils induced by 0.2 mol/L sodium arsenite was higher than normal groups after 24 h-treatment, but the difference was not significant. With the increase of exposure concentration and time, the Chang liver cells survival percentage showed a decreased tendency with a time and dose dependent manner. Compared with the normal group,the apoptosis rates of Chang liver cells significantly increased at 5-100 μmol/L （P〈0.05）; and when the drug concentration increased, the apoptosis rates of Chang liver cells showed an increasing tendency with a time-dose dependent manner. Conclusion Sodium arsenite has eytotoxicity to Chang liver cells, and it can inhibit the proliferation of cells, which may be involved in the process of apoptosis.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2012年第12期1059-1062,F0003,共5页
Journal of Environment and Health
基金
国家自然科学基金(81260410)
