摘要
目的观察脐带间充质干细胞(umbilical cord mesenchymal stem cells,UC-MSC)移植对胶原诱导型关节炎(collagen typeⅡ-induced arthritis,CIA)大鼠模型血清中:血管细胞间黏附分子-1(VCAM-1)、基质细胞衍生因子-1(SDF-1)、内皮细胞生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)表达水平的影响,初步探讨UC-MSC移植对CIA大鼠炎性趋化因子的调节作用。方法采用鸡Ⅱ型胶原加完全弗氏佐剂建立CIA大鼠模型,经大鼠尾静脉注射UC-MSC;以酶联免疫吸附试验(ELISA)检测各组大鼠干预前后血清VCAM-1、SDF-1、VEGF、TNF-α水平变化。结果模型组血清VCAM-1、SDF-1、VEGF、TNF-α水平均明显高于同期空白对照组(P<0.05);经UC-MSC治疗组上述炎症因子水平逐渐降低,至第5周上述指标均接近空白对照组(P<0.05)。结论 CIA大鼠存在免疫功能紊乱,其炎症趋化因子水平显著增高;UC-MSC能下调CIA大鼠炎症趋化因子水平,减轻机体免疫性炎症反应,促进疾病缓解,改善预后。
Objective To observe the immunomodulation effects of umbilical cord mesenchymal stem cells （UC-MSC） on the VCAM- 1, SDF- 1, VEGF, TNF- α of rats with collagen Ⅱ induced arthritis （CIA）. Methods First,The rats were immunized by intradermal injection of chickencollagen type 11 emulisified with complete Freund＇ s adjuvant （CFA）. The swelling of foot , hair color , and action state of the rats were observed after injecting UC-MSC by caudal vein,the rats were scored with the arthritis index（AI） once a week. Then, The CD 4, CD 25 of peripheral blood lymphocytes and the CD 11 b of peripheral blood neutrophil were detected by flow cytometry（FCM） ;The concentration of IL-17 in serum was detected by ELISA. Results Obvious swelling of the feet were found in the experiment group compared with norma group. ELISA the con- centration of VCAM- 1, SDF- 1, VEGF, TNF-α in serum of the experiment group are higher than normal group （ P 〈 0.05 ）. After UC- MSC treatment, these factors level were significantly difference than the model group （ P 〈 0.05 ）, and till to the fifth week these factors in the UC- MSC group were mostly the same as normal group. Conclusion UC-MSC can inhibit the development of CIA rats＇disease and reduce the immunity inflammatory reaction, to induce immunological reconstitution. UC-MSC also can lighten the CIA rats joint damage by repairing the vascular endothelial cells and synovial cells.
出处
《血栓与止血学》
2012年第6期247-250,共4页
Chinese Journal of Thrombosis and Hemostasis
基金
江苏省卫生厅2010年立项资助项目(项目编号:H201048)
关键词
脐带间充质干细胞
胶原诱导型关节炎大鼠
趋化因子
免疫调节
Umbilical cord mesenehymal stem cells （UC-MSC ）
Collagen Ⅱ induced arthritis （CIA）
Inflammatory chemokines
Immunomodulation
