摘要
目的揭示活血药、益气活血药在Lewis肺癌转移过程中差异的分子机制,为中药逆转免疫逃逸提供实验依据。方法观察各组小鼠肺转移抑制率;流式细胞术检测各组小鼠脾CD+4T淋巴细胞Th17/Treg细胞百分比动态变化;ELISA法检测各组小鼠CD+4T淋巴细胞培养上清中白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、γ干扰素(IFN-γ)动态水平;RT-PCR检测各组小鼠CD+4T淋巴细胞Foxp3、RORγt mRNA动态表达水平。结果从各组药物抑制肺转移率看出,益气活血药苏木加黄芪优于单纯活血药苏木(P<0.05),与环磷酰胺组相似(P>0.05);除苏木黄芪组外,各荷瘤组小鼠脾Th17与Treg细胞随时间迁移呈现出上升趋势;Th17与Treg细胞的效应因子IL-17、IL-23、IFN-γ以及Th17与Treg细胞关键转录分子RORγt、Foxp3呈现出与Th17和Treg细胞相应的动态改变。结论益气活血药苏木加黄芪组在抑制肿瘤免疫耐受的形成和肿瘤形成过程中的免疫炎症反应优于单纯活血药苏木组和环磷酰胺组;各组药物对肿瘤转移机制均有一定的抑制作用,但益气活血药优于单纯活血药和化疗药物。
Objective To reveal the different molecular mechanisms between Chinese drugs for activating blood （CDAB） and Chinese drugs for nourishing qi and activating blood （CDNQAB） in the metastasis process of Lewis lung carcinoma, thus providing experimental reliance for Chinese drugs to reverse immune escape. Methods The inhibition rate of lung metastasis was observed in each group. The dynamic percentage and ratio changes of Th17 and Treg cells in spleen CD4 T lymphocytes were detected using flow cytometry. The dynamic levels of IL-17, IL-23, and y interferon （IFN-y） in the culture supernatant of CD4^＋ T lymphocytes were detected by ELISA. The dynamic mRNA expressions of Foxp3 and RORyt in CD4 T lymphocytes were detected by RT-PCR. Results CDNQAB （sapanwood ＋astragalus） showed better lung metastasis inhibiting rate than CDAB （sapanwood alone） （P 〈0. 05）, similar to the effects of cyclophosphamide （P〉O. 05）. Except the CDNQAB group, spleen Th17 and Treg cells showed a rising tendency in mice of each tumor-bearing group. The effectors of Th17 and Treg cells （IL-17, IL-23, and IFN-γ） and key transcription molecules of Th17 and Treg cells （RORyt and Foxp3） showed dynamic changes corresponding to Th17 and Treg cells. Conclusions The immune inflammatory reactions of CDNQAB （sapanwood ＋astragalus） were superior to those of CDAB （sapanwood alone） and of cyclophosphamide during the process of inhibiting tumor immunotolerance and of the formation of tumor. All drugs showed certain inhibition on the mechanisms for neoplasm metastasis. But CDNQAB was superior to CDAB and chemotherapeutics.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第12期1638-1641,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81072970)
关键词
肿瘤
调节性T细胞
辅助性T细胞17
益气活血药
活血药
Lewis lung carcinoma
regulatory T cell
T helper 17 cell
drugs for nourishing qi and activating blood
drugs for activating blood
