摘要
利用表面等离子共振光学生物免疫传感器,在金膜芯片表面固定抗原技术,实现了磺胺二甲嘧啶药物的定量检测。介绍了抗原分子膜的生长方法,当配置样品中磺胺二甲嘧啶浓度分别为0,1.2,2.4,8,11.5 mg/L,与工作浓度的抗体混合,测量了传感的信号强度值,得到抑制标准曲线。在无抗牛奶中添加系列质量浓度的磺胺二甲嘧啶样品,利用免疫竞争抑制原理构建标准曲线,检测的浓度线性范围为3～48 mg/L,对浓度为40 mg/L的样品,检测的相对标准偏差小于10%,回收率为99%。检测结果表明:基于表面等离子共振的光学生物免疫传感器在磺胺二甲嘧啶药物残留方面具有很好的应用前景。
The surface plasmon resonance （SPR） optical bio-immune sensor is used for detecting the sulfamethazine residues, and it is based on the binding value between monoclonal antigen and antibody of sulfamethazine on the sensor chip surface, which can be used for the capability of quantitative detection for sulfamethazine. The binding values are measured as the concentrations of sulfamethazine are 0,1. 2,2. 4,8, 11.5 mg/L. The standard curve for sulfamethazine residue in milk is established, and which the linear range for samples is 3 ~ 48 mg/L. The relative standard deviation is less than 10 % and percent recovery is 99 % for 40 mg/L concentration. It is sure that the optical bio-immune sensor based on the SPR can be applied in detection of sulfamethazine residues in milk.
出处
《传感器与微系统》
CSCD
北大核心
2012年第12期133-135,共3页
Transducer and Microsystem Technologies
基金
海南省重点科技基金资助项目(ZDXM20100099)
海南师范大学重点学科项目(HS-2-2011-070207)
