期刊文献+

SPR生物传感器快速检测醋酸甲羟孕酮的初步应用研究 被引量:5

Preliminary Application of Surface Plasmon Resonance(SPR)-Based Biosensor Immunoassay on Detection of Medroxy Progesterone Acetate
下载PDF
导出
摘要 动物源性食品中残留的醋酸甲羟孕酮(medroxy progesterone acetate,MPA)对人类健康有潜在的危害。利用SPR生物传感器快速检测MPA,对抗原的固定条件、抗体的浓度及芯片再生条件进行了优化,同时对抗体的稳定性及特异性进行了分析。结果显示,该方法检测的最低限约为1 ng/mL。利用模拟标本进行平行测试表明,其与进口ELISA检测试剂盒结果基本一致。SPR生物传感器检测MPA,单个样品的检测时间小于5 min,非常适合现场的快速检测,具有广泛的应用前景。 The residues of medroxy progesterone acetate(MPA) in food of animal origin have potential hazards to people's health.A sensitive and rapid immunoassay of a surface plasmon resonance(SPR)-based biosensor was developed for detecting MPA residues,the conditions for immobilizing MPA antigen on the chip were explored,and the reactive concentration of antibody and the conditions for regenerating chip were optimized.The stability and the specificity of the antibody were also evaluated.The results showed that the lowest detective threshold value of SPR-based biosensor immunoassay was about 1 ng/mL MPA.In a parallel assays performed with simulation samples,its detective results were concordance with those of MPA EIA Kit imported.The detective time needed for a single sample was less than 5 min using the SPR-based biosensor.Therefor,it's very suitable for rapid detection on-site and would be have a broad prospect of application.
出处 《中国畜牧兽医》 CAS 北大核心 2010年第3期102-105,共4页 China Animal Husbandry & Veterinary Medicine
基金 国家质检总局科研项目(2006IK017)
关键词 醋酸甲羟孕酮 SPR生物传感器 快速检测 medroxy progesterone acetate surface plasmon resonance(SPR)-based biosensor rapid detection
  • 相关文献

参考文献7

二级参考文献31

  • 1张微微,陈宇春,王菊英,马德毅.表面等离子体共振技术在环境污染物分析中研究[J].环境科学与技术,2007,30(5):95-97. 被引量:10
  • 2GIUSEPPE C,MICHAEL P,SHWAN R,et al.Development of an optical biosensor assay for detection of β-lactam antibiotics in milk using the penicillin-binding protein 2x*[J].Analytica Chimica Acta,2004,520(1-2):105-115. 被引量:1
  • 3INDYK H E,FILONZI E L.Determination of lactoferrin in bovine milk,colostrum and infant formulas by optical biosensor analysis[J].International Dairy Journal,2005,15(5):429-438. 被引量:1
  • 4WU C M,LIN L Y.Immobilization of metallothionein as a sensitive biosensor chip for the detection of metal ions by surface plasmon resonance[J].Biosensors and Bioelectronics,2004,20(4):864-871. 被引量:1
  • 5STERNESJO A,MELLGREN C,BJORCK L.Determination of sulfamethazine residues in milk by a surface plasmon resonance-based biosensor assay[J].Analytical Biochemistry,1995,226(1):175-181. 被引量:1
  • 6AHMAD A,RAMAKRISHNAN A,MCLEAN M A.Use of optical biosensor technology to study immunological cross-reactivity between different sulfonamide drugs[J].Analytical Biochemistry,2002,300(2):177-184. 被引量:1
  • 7AKKOYUN A,KOHEN V F,BILITEWSKI U.Detection of sulphamethazine with an optical biosensor and anti-idiotypic antibodies[J].Sensors and Actuators B:Chemical,2000,70(1-3):12-18. 被引量:1
  • 8BJURLING P,BAXTER G A,CASELUNGHE M,et al.Biosensor assay of sulfadiazine and sulfamethazine residues in pork[J].Analyst,2000,125:1771-1774. 被引量:1
  • 9HAASNOOT W,BIENENMANN-PLOUM M,KOHEN F.Biosensor immunoassay for the detection of eight sulfonamides in chicken serum[J].Analytica Chimica Acta,2003,483(1-2):171-180. 被引量:1
  • 10BIENENMANN-PLOUM M,KORPIM(A)KI T,HAASNOOT W,et al.Comparison of multi-sulfonamide biosensor immunoassays[J].Analytica Chimica Acta,2005,529(1-2):115-122. 被引量:1

共引文献19

同被引文献54

引证文献5

二级引证文献5

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部