摘要
为提高油莎豆油提取率和油品品质,以油莎豆为原料,以油莎豆油提取率及理化性质为指标,借助气相色谱/质谱联用仪(GC-MS),比较分析溶剂法、水酶法、水酶法-冻融耦合技术3种提油方法的效果。并通过单因素试验和正交试验,优化了水酶法-冻融耦合技术提取油莎豆油的工艺。结果表明,采用碱性蛋白酶和纤维素酶复合酶,水酶法提取的最佳工艺参数为:料液比1∶7(g∶mL),酶添加量2.5%,酶解温度70℃,酶解时间6 h,然后经-30℃冷冻、室温融解、4 000 r/min离心分离20 min,在此提取条件下,油莎豆油提油率达74.92%,比单纯水酶法高,而且油脂品质较优。
In order to improve the extraction efficiency and quality of Cyperus esculentus oil , three kind of oil ex- traction techniques such as solvent method, aqueous-enzyme method and aqueous-enzyme and freeze-thaw coupling technique were compared using gas chromatography/mass spectrometry （GC-MS） with oil-extraction rate and physical- chemical properties as index. A new preparation technology of Cyperus esculentus oil was developed using aqueous-en- zyme and freeze-thaw coupling technique. The technological parameters of aqueous-enzyme and freeze-thaw coupling technique were optimized through single factor experiment and orthogonal test. The results showed that the most appro- priate conditions in the process of enzymolysis were as follows： alkali protease and cellulose enzyme composite enzyme were adopted, ratio of material to solvent was 1： 7, enzyme amount was 2.5% of dried Cyperus esculentus powder weight, temperature was 70℃ and enzymolysis time was 4h, then it was frozen at -30℃, molten at room temperature, centrifu- galized at 4 000r/min for 20min. Under these conditions, the oil extraction rate was up to 74.92% , which was higher than aqueous-enzyme method. The quality of oil obtained by aqueous-enzyme and freeze-thaw coupling technique was the best. The aqueous-enzyme and freeze-thaw coupling technique was regarded as the ideal extraction process of Cype- rus esculentus oil. This paper can offer some reference for industrial production of Cyperus esculentus oil.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第10期182-188,共7页
Food and Fermentation Industries
基金
新疆生物资源基因工程重点试验室资助(XJDX0201_2011_09)
关键词
优化
提取
油莎豆油
水酶法-冻融耦合技术
optimization, extraction,processing, Cyperus esculentus oil, aqueous-enzyme and freeze-thaw coupling technique
