摘要
目的:探讨经典Wnt通路在骨髓间充质干细胞向成牙本质样细胞分化中的作用,以期为临床治疗牙髓损伤提供新策略。方法:用牙胚细胞条件培养液诱导骨髓间充质干细胞向成牙本质样细胞的分化,培养液内分别加入外源性Wnt激活剂Wnt3a/抑制剂DKK-1,检测经典Wnt通路中β-catenin、LEF1、TCF4及成牙本质样细胞特异表达物DSPP、DMP-1变化。结果:骨髓间充质干细胞经Wnt激活剂/抑制剂处理后,胞浆内β-catenin水平明显上升/下降,其下游核内转录因子LEF1、TCF4基因水平明显上升/下降,而细胞内DSPP、DMP-1的表达明显降低/升高。结论:Wnt信号通路抑制骨髓间充质干细胞向成牙本质样细胞分化。
Objective： To study the effect of the canonical Wnt signalling on the odontoblast-like differentiation of bone marrow mesenchymal stem cells （ BMMSCs ） in vitro. Methods ： Tooth germ cell conditioned medium was used to induce the differentiation of BMMSCs to odontoblast-like cells, the effect of canonical Wnt signaling on the odontogenic differentiation of BMMSCs was determined by treating the cells with Wnt3a and DKK-1 （inhibitor of Wnt3a） respectively. β-catenin, LEF1, TCF4 DSPP and DMP-1 expressions were examined by immunofluorescence staining, western blot and RT-PCR. Results： After treatment by Wnt3a or DKK-1 and followed by tooth germ conditioned culture medium（TGC-CM） , the expression of β-catenin, LEF1, TCF4 was significantly up-regulated or down-regnlated, while the level of DSPP, DMP- 1 was decreased or increased. Conclusion ： Wnt signalling pathway negatively regulates the odontoblast-like differentiation of BMMSCs.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2012年第6期673-676,共4页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:30870636)
