摘要
目的:克隆人成牙本质细胞L型钙离子通道α1亚基D亚型的特异性基因。方法:采用RT-PCR方法,从人成牙本质样细胞系中克隆人成牙本质细胞L型钙离子通道α1亚基的特异性基因片段,将其亚克隆入pUC18载体进行序列测定。结果:从人成牙本质样细胞系中获得900bp的特异性片段。序列分析表明,与Gen-Bank登陆的人L型钙离子通道α1亚基D亚型的特异性基因序列完全一致。结论:成功地从人成牙本质样细胞系中克隆到人成牙本质细胞L型钙离子通道α1亚基D亚型的特异性基因序列。
Objective：To clone L type calcium channel α1 D subunit specific gene of human odontoblasts. Methods： L type calcium channel α1 D subunit specific gene of human odontoblasts was cloned from human odontoblast-like cell line by RT-PCR, target fragment was inserted into pUC18 vector and sequenced. Results：A 900 bp fragment was obtained from human odontoblast-like cell line. The sequence analysis showed that it was 99.9% homologous to human L type calcium channel α1 D subunit specific gene reported by Genebank. Conclusion： The specific sequence of L type calcium channel α1 D subunit of human odontoblast is successfully cloned from human odontoblast-like cell line.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第3期347-349,共3页
Journal of Practical Stomatology
基金
国家自然科学基金资助项目(资助号:30471889)
