摘要
目的:探讨近红外荧光分子标记的凝集素样氧化低密度脂蛋白受体-1(LOX-1)抗体探针在ApoE-/-鼠主脉动脉粥样硬化近红外荧光(NIRF)成像中的可行性。方法:将ApoE-/-小鼠以高脂饮食喂养20周,建立动脉粥样硬化模型。将24只ApoE-/-动脉粥样硬化模型小鼠随机分为3组,即抗LOX-1抗体-NIR-797组、非特异性IgG-NIR-797组和单纯磷酸盐缓冲液(PBS)组,每组8只,分别经尾静脉注入抗LOX-1抗体-NIR-797、非特异性IgG-NIR-797和单纯PBS。另取8只C57BL/6小鼠作为对照组,经尾静脉注入抗LOX-1抗体-NIR-797,注入探针24 h后取小鼠主动脉及主要脏器行NIRF成像,再取主动脉行油红O染色,比较NIRF成像面积和油红O染色面积比例的差异。结果:ApoE-/-小鼠经尾静脉注入抗LOX-1抗体-NIR-797探针24 h后,NIRF成像显示有强荧光信号聚集于小鼠离体主动脉的斑块区域,而注入非特异性IgG-NIR-797组斑块区信号明显减弱,PBS组及对照组C57BL/6小鼠仅见较弱荧光信号,单因素方差分析结果显示差异有统计学意义(F=232,P<0.001)。NIRF阳性面积与斑块油红O染色面积比例分别为(42.70±1.25)%和(42.78±1.51)%,两者差异无统计学意义(t=0.409,P=0.695)。结论:近红外荧光分子标记的抗LOX-1抗体探针能够特异性地显示ApoE-/-鼠主动脉粥样硬化斑块的分布,可以作为小鼠动脉粥样硬化斑块成像的有效工具,LOX-1将来可能成为一个新的评价动脉粥样硬化的良好靶点。
Objective： To explore the role of near-infrared fluorescence （NIRF） probes NIR-797 targeted anti-LOX- 1 antibodies in atherosclerotic lesions imaging in aortic atherosclerosis lesions of ApoE deficient mice. Methods：Apolipoprotein E -/- （ApoE -/- ） mice were fed with high cholesterol diet for 20 weeks to establish atherosclerotic model. Twenty-four ApoE -/- mice were randomly divided into 3 groups（8 in each group） and injected with anti- LOX- 1- Ab- NIR- 797, IgG- NIR- 797 and phosphate buffered saline （PBS） respectively. Eight wild- type C57BL/6 mice were injected with anti-LOX-1-Ab-NIR-797 as control group. NIRF imaging of the aortas and major organs were performed 24 hours after tail vein injection of probes, followed by aortic excision for oil red O staining. Reults： NIRF imaging showed significant fluorochrome enhancement accumulation in atherosclerotic plaques in ApoE -/-mice with the anti-LOX-1-Ab-NIR-797 probe but not enough with the nonspecific IgG probe. Little signal enhancement was observed in ApoE-/- mice injected with the PBS and Wild-type C57BL/6 mice injected with anti-LOX-1-Ab-NIR- 797 probe. The percentage of positive areas in imaging of NIRF and oil red O staining 24 h after injection of NIR-797 labeled anti- LOX- 1 antibody were （42.70 ± 1.25 ） % and （42.78±1.51 ） %. The difference between them was not statistically significant （ t = 0.409, P = 0. 695 ）. Conclusion： This study demonstrates that anti- LOX- 1- Ab- NIR-797 can be used in imaging atherosclerotic lesions. LOX-1 may be used as a target for molecular imaging of atherosclerotic plaque.
出处
《东南大学学报（医学版）》
CAS
2012年第6期665-670,共6页
Journal of Southeast University(Medical Science Edition)
基金
国家自然科学基金(30910103905)
