摘要
目的研究牙根发育不同阶段的恒牙牙髓中Notch1蛋白的表达,探讨其在牙髓发育和成熟中的作用。方法收集因正畸而拔除的人健康恒牙,拔除后立即取出牙髓,固定,石蜡包埋。按照牙根发育程度,分为3组:第1组(牙根刚开始发育),牙根发育不足1/3,共10例;第2组(牙根发育中),牙根发育1/3～2/3,共15例;第3组(牙根发育完成),根尖孔闭合,共12例。采用SP法,对牙髓标本的石蜡切片进行Notch1的免疫组化染色。利用图像分析仪和Meta Morph/Cool snapfx/AX70软件系统对阳性染色部位的透光强度进行定量分析。应用SPSS 10.0软件包,采用方差分析进行统计学分析。结果 Notch1蛋白在恒牙牙根发育不同阶段的牙髓组织中均有表达,主要在牙髓成纤维细胞和血管内皮细胞的胞质内呈阳性着色;随着牙根的逐渐发育,Notch1在牙髓中的表达强度逐渐减弱(P<0.01)。结论 Notch1参与牙髓牙本质复合体的发育和成熟。
Objective To investigate the expression of Notchl protein in dental pulp of permanent teeth at different root develop- ment stages and to explore its possible role in the development and maturation of dental pulp. Methods Healthy permanent teeth in need of orthodontic extraction were obtained. The dental pulp was immediately removed after extraction, fixed and embedded in paraffin. Based on the teeth root development statutses, the pulp tissues were classified into three groups. In Group 1,the tooth roots just started their development, and their development was less than 1/3. In Group 2, the tooth roots in development had formed 1/3 to 2/3. In Group 3, the tooth roots formed fully （apical closed）. The sample numbers in each group were 10,15 and 12 respectively. The dental pulp was immunohistoehemically stained with Notchl antibody with streptavidin-peroxidase method. Meta Morph/Cool snapfx/AX70 image analy- sis softwares and image analyzer were used for quantitative analysis of the intensity of positive staining. The data were statistically ana- lysed by ANOVA using SPSS 10.0 software package. Results Notehl protein expressed in the dental pulp of permanent teeth at dif- ferent root development stages. Positive staining was observed in the cytoplasm of fibroblasts and vascular epithelial cells. The expression intensity of Notchl in the dental pulp became lower and lower with the gradual formation of tooth roots （ P 〈 0.01 ）. Conclusions Notchl could be involved in the development and maturation of pulp-dentin complex.
出处
《口腔医学》
CAS
2012年第11期643-645,共3页
Stomatology
基金
辽宁省教育厅科学技术研究项目(2009A750)
关键词
NOTCH1
牙髓
牙齿发育
年轻恒牙
免疫组化
Notchl
dental pulp
tooth development
immature permanent tooth
immunohistochemistry
