摘要
目的:研究荞麦花叶提取物(extraction of buckwheat flower and leaf,EBFL)对肿瘤的抑制作用及其对荷瘤鼠体内抗氧化能力的影响。方法:建立S180小鼠实体瘤和腹水瘤模型,80只昆明种小鼠随机分为4组:模型组、EBFL 200,400 mg·kg-1剂量组ig给药、环磷酰胺(CTX,25 mg·kg-1)ip组。所有动物每日给药1次,连续给药20 d,测定EBFL给药后各处理组小鼠实体瘤重,腹水瘤小鼠体重和腹围增长速度,治疗组和对照组观察时间结点为25 d,计算各组平均生存率,以及全血谷胱甘肽过氧化物酶(GSH-Px)及血浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:EBFL处理15 d,荷瘤小鼠腹水增长、体重增长明显减慢,与模型组比较P<0.05。EBFL处理组腹水瘤小鼠生存率达25%。EBFL对肿瘤的生长有一定的抑制作用,但仍未达到CTX的水平。与对照组相比,EBFL(L,H)均可升高荷瘤小鼠血清中GSH-Px活性(P<0.05,P<0.01);EBFL(H)组小鼠血清SOD活性与对照组相比显著升高(P<0.05),作用效果与CTX相似;与对照组相比,EBFL(L,H)处理均可以显著降低小鼠血清MDA水平(P<0.05)。结论:荞麦花叶提取物对S180荷瘤小鼠有一定的抑制作用,能抑制荷瘤小鼠腹水生长,延长荷瘤小鼠生存率,这可能与提高实验小鼠体内的GSH-Px和SOD的活性,降低MDA含量有关。
Objective： To explore the antioxidative and antitumor effects of buckwheat flower and leaf extracts on S180 tumor-bearing mice. Method： The mice S180 tumor model was established; the model groups were divided into extraction of buckwtheal flower and leaf （EBFL） -low group （200 mg ·kg^-1） , EBFL-high group （400 mg ·kg^-1）, and combined with cydophosphamide （CTX, 25 mg ·kg^-1 ）. The solid tumor weight, ascites tumor weight and abdominal circumference growth rate and survival rate were measuredafter administration. glutathione peroxidase （GSH-Px）, superoxide dismutase （SOD） activity and malondialdehyde （MDA） content were determined. Result： After EBFL treatment for 15 d, the symptom of body weight loss was ameliorated and the growth of tumor was inhibited in tumor-bearing mice. EBFL （L, H） treatment could increase the levels of serum GSH-Px （P 〈 0.05, P 〈 0. O1 ） and the activity of SOD in EBFL （H） group was obviously increased （P 〈 0.05） compared with those of control group. The effect was similar to CTX. Compared with control group, the level of MDA was decreased significantly in tumor-bearing mice. Conclusion： EBFL can inhibit the growth of S180 mouse in a certain extent, which may be related to the increasing GSH-Px and SOD activity and decreased MDA content.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期176-179,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省卫生厅2012年度医学科学重点项目(20120402)
河北省中医药管理局科研计划项目(2012045)
唐山市科技攻关项目(111302025b)
关键词
荞麦花叶提取物
抗肿瘤
抗氧化
自由基
extraction of buckwheat flower and leaf
antioxidative
free radical
antitumor
