摘要
目的:研究文昌鱼多糖(PBB)的组成及其对荷S180小鼠淋巴细胞增殖和抗氧化活性的影响。方法:以酶水解法对文昌鱼多糖进行提取,并应用沉淀法对粗制品进行分离纯化;以苯酚-硫酸法测定总糖含量。样品经盐酸-甲醇醇解成单糖,采用标准对照法及内标法进行气相色谱定性定量测定。建立S180荷瘤鼠模型,随机分为PBB低、中、高剂量组(25、50、100mg/(kg.d))、正常对照组、肿瘤对照组和环磷酰胺对照组,测定PBB干预对荷S180小鼠抑瘤率的影响;MTT法检测PBB对荷S180小鼠脾淋巴细胞增殖的影响;酶学实验检测PBB对荷S180小鼠血浆超氧化物歧化酶(SOD)、还原性谷胱甘肽(GSH-Px)活性及丙二醛(MDA)含量的影响。结果:PBB总糖含量为89.3%;PBB中单糖含量分别为鼠李糖1.04%、木糖1.01%、岩藻糖1.02%、甘露糖0.35%、葡萄糖0.56%、半乳糖2.64%。经25、50、100mg/(kg.d)剂量作用2周后,小鼠S180肉瘤生长均受到抑制,抑瘤率分别达到39.1%、47.5%、53.4%;荷S18 0小鼠淋巴细胞增殖率较肿瘤对照组明显提高,经统计学处理,有显著性差异(P<0.05);荷S180小鼠血浆MDA含量均显著降低,荷S180小鼠血浆SOD和GSH-Px活性明显升高,与肿瘤对照组比较,具有显著性差异(P<0.05)。结论:文昌鱼中含有丰富的多糖物质。文昌鱼多糖可明显抑制S180肉瘤的生长,其作用机制可能与PBB提高机体淋巴细胞增殖,改善机体抗氧化能力有关。
Objective： To explore the monosaccharide composition of polysaccharides extracted fromBranchiostoma belcheri （PBBs）, and the effect of PBBs on lymphocyte proliferation and antioxidant activity in S180-bearing mice. Methods： Polysac- charides were isolated from Branchistoma belcheri by enzymatic hydrolysis and alcohol precipitation. The monosaccharides obtained by alcoholysis with HCI-CH3OH from PBBs were determined by gas chromatography with internal standard method. S 180-bearing mouse models were established and the model mice were randomly divided into six groups including model group, normal group, PBBs groups （25, 50, 100 mg/（kg · d）） and cyclophosphamide group. The tumor inhibition rate of S 180-bearing mice was determined. The effect of PBBs on spleen lymphocyte proliferation was evaluated by MTT assay. The contents of SOD, MDA and GSH-Px in mouse plasma were determined using test kits. Results： The purity of PBBs was determined to be 89.3%. The neutral monosaccharides included rhamnose, xylose, fucose, mannose, glucose and galactose. PBBs administration at daily dosages of 25, 50, 100 mg/kg for 2 consecutive weeks could inhibit the growth of S 180 sarcoma with inhibitory rates of 39.1%, 47.5% and 53.4%, respectively. Meanwhile, PBBs revealed an obvious reduction in MDA content and a significant increase in plasma SOD and GSH-Px activities. Conclusion： Branchiostoma belched is rich in PBBs that function to inhibit the growth of S 180 sarcoma through enhancing spleen lymphocyte proliferation and antioxidant activity in S 180-bearing mice.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第17期249-252,共4页
Food Science
基金
山东省科技攻关计划项目(J08LH53)
关键词
文昌鱼多糖
抑瘤率
淋巴细胞增殖
抗氧化
polysaccharide from Branchiostoma belcheri
tumor inhibition rate
lymphocyte proliferation
antioxidant activity
