摘要
目的建立三七总皂苷的大孔吸附树脂纯化工艺方法,并对总皂苷进行质量分析。方法以三七总皂苷中4种主要皂苷类成分的转移率为评价指标,优选大孔吸附树脂纯化工艺条件;采用RP-HPLC法测定总皂苷中4种主要皂苷类成分的量,并建立HPLC指纹图谱;色谱条件:Kromasil C18色谱柱(250 mm×4.6 mm,5μm),乙腈-水线性梯度洗脱,体积流量1 mL/min,柱温30℃,检测波长203 nm。结果三七总皂苷得率约10%,其中含三七皂苷R1,人参皂苷Rg1、Re和Rb1的总量大于69%。HPLC指纹图谱中共确定14个特征峰,主要色谱峰分离良好。以峰1为参照峰(S),计算14个特征峰的相对保留时间和相对峰面积,各色谱峰精密度、稳定性和测定重复性RSD均小于1.64%。结论本工艺皂苷类成分转移率高,工艺简便,提取物得率高、纯度好;HPLC定量测定和指纹图谱测定方法准确,重现性良好,可用于准确评价总皂苷提取物的质量。
Objective To establish a purification method for total saponins in Panax notoginseng （TSPN） with macroporous resin and analyze their quality. Methods The purification method with macroporous resin was optimized by transfer rates of four key saponins in P notoginseng. RP-HPLC method was used to determine the content of the four saponins and to obtain the fingerprint. The HPLC conditions were as follow： Kromasil Cl8 column （250 mm x 4.6 mm, 5 μm）, linearity gradient elution with acetonitrile and water, flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength at 203 nm. Results The yield of TSPN was about 10%, and the contents ofnotoginsenoside R1, ginsenosides Rgl, Re, and Rbl exceeded 69%. Fourteen characteristic peaks were identified and main peaks were well separated. The relative retention time and relative area of 14 peaks were calculated by taking notoginsenoside R1 peak （lst） as the reference peak. The RSD values of accuracy, stability, and repeatability were less than 1.64%. Conclusion The method is simple to operate with high transfer rate of TSPN and high yield and purity of the extract. Quantitative determination by HPLC and fingerprint is accurate and has the good reproducibility, which could be used to precisely evaluate the quality of TSPN.
出处
《中草药》
CAS
CSCD
北大核心
2012年第11期2177-2182,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重大项目资助(90209011)
国家重大新药创制专项(2009ZX09103-355)
关键词
三七
三七总皂苷
大孔吸附树脂
高效液相色谱
指纹图谱
Panax notoginseng （Burk） F. H. Chen
total saponins in Panax notoginseng （TSPN）
macroporous resin
HPLC
fingerprint
