摘要
近几年来,浙江省宁波市区的黄山栾树发生严重的裂皮病,主干树皮和韧皮部大量开裂、龟裂、变褐色,随后大块脱落,裸露的木质部也变为褐色。病害严重的树木,春季新梢发生少、夏季叶片萎蔫,发病严重时树木枯死。以变褐木质部为材料,对病菌进行分离。通过田间致病性测定以及人工接种后再分离病菌;证明编号为KL-1-2的真菌菌株为该病的致病菌;该病菌在PDA上菌落边缘不整齐,初期灰白色,后转为墨绿色或黑色;菌丝发达,绒毛状,有分隔,不规则分支;分生孢子椭圆形或卵形,初期单胞无色,老熟后变褐色,近中部有一横隔;分生孢子大小为(23.2～27.0)μm×(10.8～16.2)μm(平均25.3μm×13.6μm)。rDNAITS序列分析结果表明:该菌与可可毛色二孢同源性为99%。运用形态学及ITS-rDNA序列分析方法对菌株KL-1-2进行鉴定,确定为可可毛色二孢。
A disease on Koelreuteria bipinnata var.integrifoliola was reported in Zhejiang Province in recent years.The disease caused a series symptoms,beginning with a serious bark including phloem crack in stem,later the cracked bark largely shed off and the exposed xylem turned brown.Trees with serous lesions produced less new shoots in spring,wilted and then died in summer.The suspected pathogen was isolated from diseased phloem.After pathogenicity tests in field and re-isolation of the pathogen,the strain KL-1-2 was determined to be responsible for the disease.The fungus colony on PDA was circular or nearly circular in shape with irregular edge and gray,and then turned to black green or black.The fungus had abundant hyphae,villous,septate and irregular branched.Conidia were elliptical（or rounded） and hyaline at the stage of immature.However,conidia became dark brown and septate longitudinally at the stage of mature.Mature conidia size was ranged from（23.2-27.0） μm×（10.8-16.2） μm [average（25.3×13.6） μm].Its rDNA ITS sequence had 99% similarity with those of Lasiodiplodia theobromae（Patouillard） Griffon=Botryodiplodia theobromae Pat.Morphological characteristics and internal transcribed spacer（ITS） sequences show that the strain KL-1-2 would be Lasiodiplodia theobromae.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2012年第10期95-100,共6页
Scientia Silvae Sinicae
基金
宁波市镇海区科技局计划项目"宁波市镇海区园林植物病虫害无公害防治技术研究"(2069999-53)
关键词
黄山栾树
裂皮病
病原
可可毛色二孢
Koelreuteria bipinnata var.integrifoliola
bark cracking
pathogen
Lasiodiplodia theobromae
