摘要
以MT为基本培养基并附加各种植物激素和生理活性物质的一系列培养基中,8周龄的“华盛顿脐橙”(Citrus sinensis Osbeck)未受精胚珠,通过体细胞胚胎发生或器官发生再生出完整植株。外源激素的种类和组合对愈伤组织的诱导、生长状况和分化有显著的影响。指示植物鉴定证明,已从感病母株中获得了脱去裂皮病和衰退病的无病毒珠心苗。
On a series of Murashige and Tucker (MT) media supplemented with different growth regulators, the 8--week--old unfertilized ovules of Washington navel orange (Citrus sinensis) were able to regenerate perfect plantlets via somatic embryogenesis or organogenesis. The sorts and combinations of exogenous hormones had remarkable effects on the induction, growth and differentiation of its callus. It was found that the most suitable induction medium was MT medium supplemented with 1.0 mg/l BA and 0.5 mg/l IAA. The most suitable differentiation medium was MT medium supplemented with 1.0 mg/l IBA. It was proved by indicator plant examination that the nucellar plantlets free of citrus exocortis virus (CEV) and citrus tristeza virus (CTV) had been obtained from infected trees.
关键词
柑桔
胚珠培养
脱毒
Citrus
Ovule culture
Virus elimination
