摘要
为探索适宜切花月季快速繁殖的培养基,为切花月季种苗的大量繁殖提供理论依据,采用组织培养建立无性系的方法对3种切花月季进行了研究。结果表明:以萌芽率及芽的生长情况作为评价标准,适宜卡罗拉的诱导培养基、继代培养基和生根培养基分别为1/2 MS+6-BA 1.5mg/L+NAA 0.5mg/L(萌芽率为81%)、1/2 MS+6-BA 1.0 mg/L+NAA 0.3 mg/L(增殖系数为4.67)和1/2 MS+NAA0.5mg/L+IBA 0.1mg/L,适宜艳粉的分别为MS+6-BA 0.8mg/L+NAA 0.1mg/L(萌芽率为79%)、MS+6-BA 0.5mg/L+NAA 0.1mg/L(增殖系数为3.15)和MS+NAA 0.1mg/L+IBA 0.1mg/L,适宜雪山的分别为MS+6-BA 1.0mg/L+NAA 0.1mg/L(萌芽率为75%)、MS+6-BA 1.0mg/L+NAA 0.05mg/L(增殖系数为3.33)和1/2MS+NAA 0.5mg/L。3个品种均以蛭石为基质移栽成活率最高。
The clones of three kinds of cutting rose were constructed by tissue culture to explore the suitable culture medium for rapid propagation of cutting rose and provide a theoretical basis for mass propagation of cutting rose seedlings.The results showed that the preferable media for producing buds was 1/2 MS＋6-BA 1.5 mg/L＋NAA 0.5 mg for kalola（budding rate 81%）,MS＋6-BA 0.8 mg/L＋NAA 0.1 mg/L for pink（budding rate 79%） and MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L for snow（budding rate 75%）.The optimum multiplication medium for kalola,pink and snow were 1/2 MS＋6-BA 1.0 mg/L＋NAA 0.3 mg/L（multiplication coefficient 4.67）,MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L（multiplication coefficient 3.15） and MS＋6-BA 1.0 mg/L＋NAA 0.05 mg/L（multiplication coefficient 3.33） respectively.1/2 MS＋NAA 0.5 mg/L＋IBA 0.1 mg/L was the optimum rooting medium for kalola;MS＋NAA 0.1 mg/L＋IBA 0.1 mg/L was the optimum rooting medium for pink;1/2 MS ＋NAA 0.5 mg/L was the optimum rooting medium for snow.Transplanting survival rate was all obviously high with vermiculite as media.
出处
《贵州农业科学》
CAS
北大核心
2012年第10期18-21,共4页
Guizhou Agricultural Sciences
基金
贵州省科学技术基金项目"切花月季的微体繁殖技术研究"[黔科合J字(2011)2178]
贵州省农业科技攻关"月季不同培育目标定向栽培关键技术研究与示范"[黔科合NY字(2011)3075]
"月季品种收集
评价及应用示范"[黔科合NY字(2010)3030]
关键词
切花月季
快速繁殖
培养基
cutting rose
rapid propagation
medium
