摘要
[目的]为了快速繁育月季花的优良品种、保持品系的优良性状，最终实现月季花工厂化育苗。[方法]通过离体培养的方法对月季花进行组织培养基配方筛选试验。[结果]在月季组织培养快速繁育过程中，以腋芽为外殖体，在附加6-BA 2.0 mg/L＋NAA 0.5-1.0 mg/L＋GA 1.0 mg/L的MS培养基上，分化率为95％，并生长成为丛生苗，分化的幼芽转移到1/2 MS＋NAA 0.5-1.0 mg/L或MS＋6-BA 2.0 mg/L＋IAA 1.0-1.5 mg/L的培养基上，诱导生根，形成了具有根、茎、叶的完整小植株，成活率达95％以上，极少数的试管苗还形成了花蕾。[结论]建立了迅速规模化生产月季花的组培方法。
Objective]The aim was to fast breeding the fine varieties of Chinese rose and keep the fine character of its strain, finally realize the factory nursery of Chinese rose. [Method]In this paper, the tissue culture medium formula screening tests of Chinese rose have been carried out by using the method of in vitro culture. [Result]As a result, during the tissue culture and fast breeding process of Chinese rose, with axillary bud as outside body, the additional 6-BA 2.0 mg/L＋NAA 0.5 1.0 mg/L＋GA l. 0 mg/L on the MS culture medium, the bud differentiation rate of Chinese rose was 95%, and then it grew into clumps seedlings. Next, the germ of differentiation was transferred to the medium of 1/2 MS＋NAA （0.5 1.0 mg/L） or MS＋6-BA 2.0 mg/L ＋ IAA l. 0-1.5 mg/L in order to be induced to grow root and form a complete small plant with roots, stems and leaves, the survival rate of them was more than 95%, and a handful of tube seedlings was also formed buds. [Conclusion]The rapidly transformation method to a large-scale production of Chinese rose was established.
出处
《园艺与种苗》
CAS
2014年第5期32-34,共3页
Horticulture & Seed
基金
中国科学院"西部之光"西部博士项目(XBBS201012)
关键词
月季花
组织培养
快速繁育
Rosa chinansis
Tissue culture
Rapid propagation
