摘要
利用月季为材料，进行较大规模组培快繁技术研究，改进了表面灭菌，效果较好．ＭＳ＋ＢＡ０．５诱导腋芽萌发成枝较好；ＭＳ＋ＢＡ１．０＋ＮＡＡ０．１用于伊丽莎白及黄和平增殖较好，而ＭＳ＋ＢＡ２＋ＮＡＡ０．１用于黄玫瑰增殖较好，萨蔓莎及维莎则用ＭＳ＋ＢＡ３＋ＮＡＡ０．１较好；ＭＳ＋ＢＡ０．１～０．２＋ＮＡＡ０．１用于各品种月季壮苗效果都理想；０．５ＭＳ＋ＩＢＡ０．２～０．５＋活性炭５ｇ／Ｌ能较好诱导各品种试管苗生根，但黄玫瑰用ＮＡＡ０．２代替ＩＢＡ效果更理想．本文还研究了培养基中替代物使用。
Seven varieties of- Rosa chinensis were used as materials to study the technique of rapid multiplication by tissue culture. We improved the method of surface sterilization and it's effect was better than ever. In our study,MS+BA0.5 is more adaptable to the growth of axillary bud , MS+BA1.5+NAA0.1 is more adaptable to shoot multilication of Elizabeth and Huang huo ping,MS+BA2+NAA0.1 is more adaptable to Yellow rose ,and MS+BA3+NAA0.1 is more adaptable to Samantha and weitha. MS+BA0.1～0.2+NAA0.1 is more adpaptable to making the shoot strong. Further more,#Owe have studied the usage of substitute in medium and expressed some viewpoints that is different from the previews.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1997年第4期366-369,共4页
Journal of Yunnan University(Natural Sciences Edition)
关键词
月季
带腋芽茎段
组织培养
快速繁殖
Rosa chinensis Jacd,Stem segment with axillary bud,Rapid multiplication by tissue culture of plant
