摘要
目的对重组胸腺素α1pMAL-C2x-Tα1/TB1工程菌的培养条件进行优化。方法在摇瓶培养条件下探讨工程菌的生长和表达规律,对pMAL-C2x-Tα1/TB1菌种的培养基装量、接种量、诱导剂异丙基硫化半乳糖苷(IPTG)加入时间和浓度、诱导时间进行了实验和优化。结果由摇瓶培养获得的数据表明pMAL-C2x-Tα1/TB1最优培养条件为:10%接种量、100 mL LB培养基、37℃培养,接种后3 h,入终浓度为0.75 mmol/L的IPTG,诱导4 h后目的蛋白表达量最高。结论优化的培养条件为菌种的发酵工艺奠定了基础。
Objective To optimize the cultivation condition for maximizing the productivity of recombinant thymosin alpha1 protein from engineering bacterial E. coli pMAL-C2x-Tαl/TB1. Methods The best cultivation and induction condition to yield the highest production were explored with the flask-shaking method. Results The data obtained from flask-shaking showed that the best expression could be achieved by cell growth, The best cultivation condition started from 10% inoculation, in 100 mL LB medium at 37 ℃ for 3 hours, followed by additional 4 hours induction with 0.75 mmol/L IPTG. Conclusion The optimal production technic of recombinant thymosin alpha 1 was established to laid a foundation to produce recombinant thymosin alpha 1 on a large scale fermentation.
出处
《实用医技杂志》
2012年第11期1128-1131,共4页
Journal of Practical Medical Techniques
关键词
胸腺素
发酵
条件优化
Thymosin
Fermentation
Conditions optimize
