摘要
目的:优选山核桃树皮中槲皮苷的提取工艺,并建立其含量测定方法。方法:采用乙醇回流法提取山核桃树皮,经聚酰胺吸附去杂、多次析晶得槲皮苷,采用核磁共振波谱法(NMR)确证其结构,HPLC测定其含量。通过正交试验考察乙醇体积分数、提取时间和提取次数对槲皮苷得率的影响。结果:经NMR确证产物为槲皮苷,HPLC表明其在4.8～91.2 mg·L-1呈良好线性关系,山核桃树皮中槲皮苷含量高达8.37%。最佳提取工艺为10倍量60%乙醇回流提取2次,每次1 h,提取率>90%。结论:建立的山核桃中槲皮苷含量测定方法简单精确,优选的提取工艺稳定可行。
Objective： To optimize extraction technology of quercitrin from bark of Carya cathayensis, and establish its content determination method. Method： Ethanol reflux extraction method was conducted, extraction solution was absorbed by polyamide to remove dopant, then condensed, crystallized and recrystallized to obtain pure product. After identification by NMR, the content of quercitrin was determined by HPLC. Orthogonal test was used to investigate influence of the concentration of ethanol, extraction time and extraction times on yield of quercitrin. Result： NMR verified product as quercitrin, HPLC showed that quercitrin had a good linear relationship in the range of 4. 8-91.2 mg-L-1, the content of quercitrin from bark of C. cathayensis was up to 8.37%. Optimum extraction technology was： reflux extracted 2 times with 10 times the amount of 60% ethanol, 1 h per time, extraction rate of quercitrin 90%. Conclusion： Established content determination method of quercitrin from C. cathayensis was accurate and simple, optimized extraction technology was stable and feasible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第22期42-45,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省"新苗"人才计划项目(2010R410002)
