摘要
研究了山核桃Carya cathayensis叶片提取物对几种常见菌种的抑制作用。采用滤纸片法,检测不同浓度山核桃叶片乙醇和水提取物对金黄色葡萄球菌Staphylococcus aureus,大肠杆菌Escherichia coli,酵母菌Saccharomyces cerevisiae,黑曲霉菌Aspergillus niger,黄曲霉菌Aspergillus flavus和青霉菌Penicillium sp.的抑制效果。结果表明,培养12 h,乙醇提取物对5个供试菌种表现出显著的抑制作用(P<0.05),其效果优于水提取物。培养24 h,乙醇提取物和水提取物对黄曲霉菌的抑制作用明显(P<0.05),而对金黄色葡萄球菌和酵母菌的抑制作用较12 h前有所降低。培养36 h,乙醇提取物对金黄色葡萄球菌和酵母菌仍保持一定的抑制作用。
Environmentally-safe pesticides extracted from plants are paid more attention. The plants of Juglandaceae have some antibiotic substances, as flavonoid, polyphenols and terpenoid. This research was to study the bacteriostasis and fungistasis from extracts of Carya cathayensis leaves. A filter paper method was used to determine whether alcohol （95%） extract or water （sterilized） extract from the leaves of C. cathayensis, diluted with sterile water in different concentrations （0.25, 0.05, 0.01, 0.002 kg· L^-1）, had an inhibitory effect on Staphylococcus aureus, Escherichia coli, Saccharomyces cerevisiae, Aspergillus niger, Aspergillus flavus, and Penicillium sp. Results indicated that after a 12 h culture inhibition with the alcohol extract on the six strains was significantly greater （ P 〈 0.05）. After a 24 h culture, inhibition on A. flavus with both the alcohol extract and water extract was significantly greater （ P 〈 0.05） than on the other bacteria and fungi. Moreover, after 24 h culture, water extract nearly had no inhibitory effect on S. aureus and S. cerevisiae than at 12 h. After a 36 h culture, however, the alcohol extract still had a significant inhibitory effect （ P 〈 0.01 ） on S. aureus and S. cerevisiae. [ Ch, 2 tab. 9 ref. ]
出处
《浙江林学院学报》
CAS
CSCD
北大核心
2007年第5期604-607,共4页
Journal of Zhejiang Forestry College
基金
浙江省自然科学基金资助项目(Y304053)
浙江省临安市科技局资助项目(05064)
关键词
植物保护学
山核桃
叶片
提取物
抑菌效果
plant protection
Carya cathayensis
leaves
extract
bacteriostasis and fungistasis
