摘要
采用凝胶板配方和电泳条件优化后的SDS-PAGE技术对酸法明胶水解物、碱法明胶水解物、胃蛋白酶法明胶水解物、胃蛋白酶法猪皮胶原水解物、明胶以及猪皮胶原蛋白透析物等进行表征,获得了将分布于一块凝胶板上的1～100kD的棒状胶原蛋白各组分的色谱图。胃蛋白酶法猪皮胶原蛋白水解物经电泳分离后的色谱图中,各谱带清晰明显,分子量均一,且均匀分布于1～100kD不等,这对棒状胶原蛋白分子的研究具有重要价值。
An attempt was made to use CB - peptides as new makers and to optimise the gel formulas and electrophoresis conditions for SDS -polyaerylamide gel electrophoresis to char^erise the acid -hydrolyzed gelatin, .alkali -hydmlyzed gelatin, pepsinhydrolyzed gelatin as well as pepsin- hydrolyzed pig skin collagen. The different components of rod - like collagen hydrolyses with molecular weight possibly ranging from I to 100 kDalton can be separated, displayed clearly in their electrophoresis scanned chromatograms and quantitatively characterised successfizlly. In a typical electrophoresls chroraatogram d pepsin hydrolyzed pig skin collagen, all the bands were dearly localised and their molecular weights orderly were shon to be dislributed between 1 and 100 kD after calibrating. All these are of important values foz the test of collagen hydrolyses.
出处
《明胶科学与技术》
CAS
2012年第3期113-126,共14页
The Science and Technology of Gelatin
