摘要
目的研究三氧化二砷(As2O3)联合肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导人肺腺癌A549细胞凋亡及对核转录因子-kappaB(NF-κB)表达的影响。方法采用As2O3、TRAIL单用和联合作用于体外培养的A549细胞,以四甲基偶氮唑蓝比色法测细胞增殖抑制率和流式细胞术检测细胞凋亡率,RT-PCR检测NF-κBmRNA的表达,Western blot检测NF-κB蛋白的表达,酶联免疫吸附(ELISA)检测NF-κB的活性。结果 As2O3与TRAIL联用对A549细胞增殖的抑制作用均比单药组强(P<0.05);联合用药组诱导凋亡作用强于单药组(P<0.05);联合用药组明显抑制NF-κB表达,并抑制其活性(P<0.05)。NF-κB mRNA、蛋白及其活性与细胞凋亡率呈负相关(P均<0.05)。结论 As2O3可能通过NF-κB通路,增强TRAIL诱导A549细胞凋亡的作用。
Objective To study the influence of arsenic trioxide combined with human tumor necrosis factor related apoptosis inducing ligand （TRAIL） on the apoptosis and the expression of NF-κB of human non-small-cell lung cancer ceil line. Methods The proliferation of human non-small-cell lung cancer cell line A549 cultured in vitro were treated by As2O3, TRAIL alone and combined. The cell proliferation was detected by the assay of MTT, flow cytometry with PI stain was used to detect the apoptosis rate, NF-κB mRNA level of A549 cells were detected by RT-PCR. The expression of NF-κB protein were detected by Western blot. The activity of NF-κB was measured by ELISA. Results Compared with As2O3 alone, As2O3 combined with TRAIL could increase the inhibition and apoptosis ratio significantly （P〈0.05）. The expression of NF-κB in combined group was obviously less than that in As2O3 alone and control; the activity of NF-κB was inhibited by combined groups. The NF-gB mRNA and protein expression and the activity of NF-κB were separately negative related with the apoptosis ratio （P 〈 0. 05）. Conclusion As2O3 can enhance TRAIL inducing of human lung cancer cell lines A549 apoptosis by inhibition of NF-κB signaling pathway.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2012年第6期834-838,共5页
Journal of Sichuan University(Medical Sciences)
基金
广西自然科学基金课题(No.0832269)资助
